MetaCyc Enzyme: ω,E,E-farnesyl diphosphate synthase

Gene: Rv3398c Accession Number: G-11697 (MetaCyc)

Species: Mycobacterium tuberculosis H37Rv

ω,E,E-farnesyl diphosphate synthase, encoded by the Rv3398c gene of Mycobacterium tuberculosis H37Rv, is a unique bacterial enzyme that has a structural motif similar to that of the type I geranylgeranyl diphosphate synthase found in Archaea.

The Rv3398c gene was cloned and expressed in Mycobacterium smegmatis, and the recombinant protein was purified to near homogeneity [Dhiman04].

From the substrates that were tested, only geranyl diphosphate served as an acceptor for isopentenyl diphosphate. The enzyme has an absolute requirement for divalent cation. Mg2+ was preferable, but Mn2+ and Fe2+ could support catalysis, at lower rates [Dhiman04].

Molecular Weight of Polypeptide: 38.852 kD (from nucleotide sequence)

Unification Links: Entrez-gene:887919 , Protein Model Portal:P9WKH0 , SMR:P0A5H8 , String:83332.Rv3398c , UniProt:P0A5H8

Relationship Links: InterPro:IN-FAMILY:IPR000092 , InterPro:IN-FAMILY:IPR008949 , InterPro:IN-FAMILY:IPR017446 , Panther:IN-FAMILY:PTHR12001 , Pfam:IN-FAMILY:PF00348 , Prosite:IN-FAMILY:PS00444 , Prosite:IN-FAMILY:PS00723

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 11-Dec-2009 by Caspi R , SRI International

Enzymatic reaction of: ω,E,E-farnesyl diphosphate synthase

EC Number:

geranyl diphosphate + isopentenyl diphosphate <=> (2E,6E)-farnesyl diphosphate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

Cofactors or Prosthetic Groups: Mg2+ [Dhiman04]

Kinetic Parameters:

Km (μM)
geranyl diphosphate
isopentenyl diphosphate

pH(opt): 8 [Dhiman04]


Dhiman04: Dhiman RK, Schulbach MC, Mahapatra S, Baulard AR, Vissa V, Brennan PJ, Crick DC (2004). "Identification of a novel class of omega,E,E-farnesyl diphosphate synthase from Mycobacterium tuberculosis." J Lipid Res 45(6);1140-7. PMID: 15060088

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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