MetaCyc Enzyme: digeranylgeranylglyceryl phosphate synthase

Gene: MTH_1098 Accession Number: G-11079 (MetaCyc)

Synonyms: (S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase

Species: Methanothermobacter thermautotrophicus Delta H

Digeranylgeranylglyceryl phosphate synthase is an integral membrane enzyme that catalyzes the transfer of a geranylgeranyl unit from geranylgeranyl diphosphate to to the C2 hydroxyl of 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate, forming 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate.

Enzymatic activity has been documented in cell-free extract of Methanothermobacter thermautotrophicus, but the protein has not been purified from that organism [Zhang90]. Orthologous enzymes have been cloned from Sulfolobus solfataricus [Hemmi04] and Methanosarcina acetivorans [Yokoi12] and the enzyme from the former was purified.

Locations: plasma membrane

Molecular Weight of Polypeptide: 30.132 kD (from nucleotide sequence)

Unification Links: Entrez-gene:1471506 , String:187420.MTH1098 , UniProt:O27170

Relationship Links: InterPro:IN-FAMILY:IPR000537 , InterPro:IN-FAMILY:IPR023547 , Pfam:IN-FAMILY:PF01040

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005886 - plasma membrane [Zhang90]

Created 23-Feb-2009 by Caspi R , SRI International

Enzymatic reaction of: geranylgeranylglycerol-phosphate geranylgeranyltransferase (digeranylgeranylglyceryl phosphate synthase)

Synonyms: (S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase

EC Number:

3-(O-geranylgeranyl)-sn-glycerol 1-phosphate + geranylgeranyl diphosphate <=> 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: archaetidylinositol biosynthesis , archaetidylserine and archaetidylethanolamine biosynthesis , Methanobacterium thermoautotrophicum biosynthetic metabolism , CDP-archaeol biosynthesis

Activators (Allosteric): Mg2+


Hemmi04: Hemmi H, Shibuya K, Takahashi Y, Nakayama T, Nishino T (2004). "(S)-2,3-Di-O-geranylgeranylglyceryl phosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked membrane lipids." J Biol Chem 279(48);50197-203. PMID: 15356000

Yokoi12: Yokoi T, Isobe K, Yoshimura T, Hemmi H (2012). "Archaeal phospholipid biosynthetic pathway reconstructed in Escherichia coli." Archaea 2012;438931. PMID: 22645416

Zhang90: Zhang, D. L., Daniels, L., Poulter, C.D. (1990). "Biosynthesis of archaebacterial membranes. Formation of isoprene ethers by a prenyl transfer reaction." J. Am. Chem. Soc. 112: 1264-1265.

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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