|Gene:||pac||Accession Number: G-10676 (MetaCyc)|
Species: Streptomyces alboniger
The subunit structure of this enzyme has not been reported.
The apparent molecular mass was determined by gel filtration chromatography [Vara85].
Molecular Weight of Polypeptide: 21.496 kD (from nucleotide sequence), 23.0 kD (experimental) [Vara85 ]
Enzymatic reaction of: N6,N6,O-tridemethylpuromycin N-acetyltransferase
Synonyms: puromycin N-acetyltransferase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is favored in the direction shown.
In Pathways: puromycin biosynthesis
The enzyme was partially purified from cell extracts of Streptomyces alboniger and its N-acetyltransferase activity was assayed using puromycin, O-demethylpuromycin and chryscandin as substrates [Vara85].
The recombinant enzyme was cloned and expressed in Escherichia coli and puromycin N-acetyltransferase activity was demonstrated [Vara85a].
Lacalle89: Lacalle RA, Pulido D, Vara J, Zalacain M, Jimenez A (1989). "Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger." Gene 79(2);375-80. PMID: 2676728
Vara85: Vara J, Perez-Gonzalez JA, Jimenez A (1985). "Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase." Biochemistry 24(27);8074-81. PMID: 4092057
Vara85a: Vara J, Malpartida F, Hopwood DA, Jimenez A (1985). "Cloning and expression of a puromycin N-acetyl transferase gene from Streptomyces alboniger in Streptomyces lividans and Escherichia coli." Gene 33(2);197-206. PMID: 3888783
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