|Gene:||dhbF||Accession Number: G-10624 (MetaCyc)|
Synonyms: dimodular nonribosomal peptide synthetase
Species: Bacillus subtilis
Alternative forms of apo-DhbF protein: holo-DhbF protein (summary available)
This protein is a very large dimodular enzyme, that contains 7 domains, responsible for glycine binding, adenylation, and condensation with 2,3-dihydroxybenzoate (DHB), L-threonine binding, adenylation, and condensation with 2,3-dihydroxybenzoylglycine, and finally, a Te domain, responsible of condensation of three DHB-glycine-threonine into a single bacillibactin molecule [May01].
The apo enzyme is activated by binding two molecules of 4'-phosphopantetheine, in a reaction catalyzed by the sfp-encoded 4'-phosphopantetheinyl transferase [May01]. The activated enzyme is referred to as holo-DhbF protein.
Molecular Weight of Polypeptide: 263.74 kD (from nucleotide sequence), 264.0 kD (experimental) [May01 ]
Relationship Links: Entrez-Nucleotide:PART-OF:AF184977 , InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR001031 , InterPro:IN-FAMILY:IPR001242 , InterPro:IN-FAMILY:IPR009081 , InterPro:IN-FAMILY:IPR010071 , InterPro:IN-FAMILY:IPR020806 , InterPro:IN-FAMILY:IPR020845 , InterPro:IN-FAMILY:IPR025110 , Pfam:IN-FAMILY:PF00501 , Pfam:IN-FAMILY:PF00550 , Pfam:IN-FAMILY:PF00668 , Pfam:IN-FAMILY:PF00975 , Pfam:IN-FAMILY:PF13193 , Prosite:IN-FAMILY:PS00012 , Prosite:IN-FAMILY:PS00455 , Prosite:IN-FAMILY:PS50075 , Smart:IN-FAMILY:SM00823
May01: May JJ, Wendrich TM, Marahiel MA (2001). "The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin." J Biol Chem 276(10);7209-17. PMID: 11112781
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