Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: desoxyhemigossypol-6-O-methyltransferase

Gene: dHG-6-OMT Accession Number: G-10459 (MetaCyc)

Species: Gossypium barbadense

Subunit composition of desoxyhemigossypol-6-O-methyltransferase = [DHG-6-OMT]2
         desoxyhemigossypol-6-O-methyltransferase = DHG-6-OMT

Summary:
The cDNA has been cloned. The sequence was shown in [Liu02], but not in GenBank.

Molecular Weight of Polypeptide: 41.2 kD (experimental) [Liu99 ]

Molecular Weight of Multimer: 81.4 kD (experimental) [Liu99]

Gene-Reaction Schematic: ?

Credits:
Created 15-Jan-2008 by Zhang P , TAIR


Enzymatic reaction of: desoxyhemigossypol-6-O-methyltransferase

EC Number: 2.1.1.-

desoxyhemigossypol + S-adenosyl-L-methionine <=> desoxyhemigossypol-6-methyl ether + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: gossypol biosynthesis

Summary:
The enzyme activity does not require metal ions. Addition of EDTA had no effects. The enzyme was strongly inhibited by the addition of 10mM p-chloromercuribenzoate (98% reduction), 10 mM iodoacetamide (63%), 5 mM heavy metal cobalt (96%), copper (98%), manganese (82%), and zinc (98%). The enzyme specifically methylates the 6-position of desoxyhemigossypol. Hemigossypol, 2,7-dihydroxycadalene, caffeic acid, 4-methylcatechol, or 2,3-dihydroxynaphthalene was not mehtylated.

Inhibitors (Unknown Mechanism): p-chloromercuribenzoate [Liu99] , iodoacetamide [Liu99] , Co2+ [Liu99] , Cu2+ [Liu99] , Mn2+ [Liu99] , Zn2+ [Liu99]

Kinetic Parameters:

Substrate
Km (μM)
Citations
desoxyhemigossypol
4.5
[Liu99]
S-adenosyl-L-methionine
81.4
[Liu99]


References

Liu02: Liu J, Benedict CR, Stipanovic RD, Magill CW, Bell AA (2002). "Cloning and expression of desoxyhemigossypol-6-O-methyltransferase from cotton (Gossypium barbadense)." J Agric Food Chem 50(11);3165-72. PMID: 12009981

Liu99: Liu J, Benedict CR, Stipanovic RD, Bell AA (1999). "Purification and Characterization of S-Adenosyl-L-Methionine: Desoxyhemigossypol-6-O-Methyltransferase from Cotton Plants. An Enzyme Capable of Methylating the Defense Terpenoids of Cotton." Plant Physiol 121(3);1017-1024. PMID: 10557251


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14B.