|Gene:||dHG-6-OMT||Accession Number: G-10459 (MetaCyc)|
Species: Gossypium barbadense
The cDNA has been cloned. The sequence was shown in [Liu02d], but not in GenBank.
Molecular Weight of Polypeptide: 41.2 kD (experimental) [Liu99e ]
Molecular Weight of Multimer: 81.4 kD (experimental) [Liu99e]
Enzymatic reaction of: desoxyhemigossypol-6-O-methyltransferase
EC Number: 2.1.1.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
In Pathways: gossypol biosynthesis
The enzyme activity does not require metal ions. Addition of EDTA had no effects. The enzyme was strongly inhibited by the addition of 10mM p-chloromercuribenzoate (98% reduction), 10 mM iodoacetamide (63%), 5 mM heavy metal cobalt (96%), copper (98%), manganese (82%), and zinc (98%). The enzyme specifically methylates the 6-position of desoxyhemigossypol. Hemigossypol, 2,7-dihydroxycadalene, caffeic acid, 4-methylcatechol, or 2,3-dihydroxynaphthalene was not mehtylated.
Liu02d: Liu J, Benedict CR, Stipanovic RD, Magill CW, Bell AA (2002). "Cloning and expression of desoxyhemigossypol-6-O-methyltransferase from cotton (Gossypium barbadense)." J Agric Food Chem 50(11);3165-72. PMID: 12009981
Liu99e: Liu J, Benedict CR, Stipanovic RD, Bell AA (1999). "Purification and Characterization of S-Adenosyl-L-Methionine: Desoxyhemigossypol-6-O-Methyltransferase from Cotton Plants. An Enzyme Capable of Methylating the Defense Terpenoids of Cotton." Plant Physiol 121(3);1017-1024. PMID: 10557251
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