|Gene:||garD||Accession Numbers: EG12522 (MetaCyc), b3128, ECK3116|
Species: Escherichia coli K-12 substr. MG1655
Enzymatic activity of galactarate dehydratase is induced by growth on D-glucarate, galactarate, and D-glycerate [Monterrubio00].
|Map Position: [3,273,304 -> 3,274,875]|
Molecular Weight of Polypeptide: 56.402 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0010284 , EchoBASE:EB2413 , EcoGene:EG12522 , EcoliWiki:b3128 , OU-Microarray:b3128 , PortEco:garD , PR:PRO_000022750 , Pride:P39829 , Protein Model Portal:P39829 , RefSeq:NP_417597 , RegulonDB:EG12522 , SMR:P39829 , String:511145.b3128 , UniProt:P39829
Relationship Links: InterPro:IN-FAMILY:IPR007392 , InterPro:IN-FAMILY:IPR013974 , InterPro:IN-FAMILY:IPR017654 , PDB:Structure:3LAZ , Pfam:IN-FAMILY:PF04295 , Pfam:IN-FAMILY:PF08666 , Smart:IN-FAMILY:SM00858
|Biological Process:||GO:0046392 - galactarate catabolic process [UniProtGOA12, GOA01, Hubbard98]|
|Molecular Function:||GO:0008867 - galactarate dehydratase activity
[GOA01a, GOA01, Hubbard98]
GO:0016829 - lyase activity [UniProtGOA11]
GO:0016836 - hydro-lyase activity [GOA01]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||metabolism → carbon utilization → carbon compounds|
Enzymatic reaction of: galactarate dehydratase
Synonyms: galactarate dehydrase, D-galactarate hydro-lyase, GalcD
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
The reaction is physiologically favored in the direction shown.
Galactarate dehydratase is catalytically inactive in the absence of Fe2+, Mn2+ and β-mercaptoethanol; however, under certain conditios, the enzyme is inhibited by Mn2+ [Hubbard98].
The enzyme was originally isolated from E. coli strain CR63MA, but is present in other strains including K-12 [Blumenthal64].
Inhibitors (Unknown Mechanism): cacodylate [Blumenthal66a] , phosphate [Blumenthal66a] , citrate [Blumenthal66a] , potassium cyanide [Blumenthal66a, Blumenthal64] , diphosphate [Blumenthal66a, Blumenthal64]
pH(opt): 7.5 [Hubbard98]
10/20/97 Gene b3128 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12522; confirmed by SwissProt match.
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Hubbard98: Hubbard BK, Koch M, Palmer DR, Babbitt PC, Gerlt JA (1998). "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Biochemistry 1998;37(41);14369-75. PMID: 9772162
Monterrubio00: Monterrubio R, Baldoma L, Obradors N, Aguilar J, Badia J (2000). "A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." J Bacteriol 2000;182(9);2672-4. PMID: 10762278
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