|Gene:||pncA||Accession Numbers: EG11135 (MetaCyc), b1768, ECK1766|
Synonyms: nam, ydjB
Species: Escherichia coli K-12 substr. MG1655
pncA in E. coli K-12 encodes a nicotinamidase that is active in the cyclical salvage pathway for production of NAD from nicotinamide [Pardee71].
PncA: "pyridine nucleotide cycle" [Tritz70]
|Map Position: [1,849,911 -> 1,850,552]|
Molecular Weight of Polypeptide: 23.362 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0005891 , DIP:DIP-10520N , EchoBASE:EB1125 , EcoGene:EG11135 , EcoliWiki:b1768 , ModBase:P21369 , OU-Microarray:b1768 , PortEco:pncA , PR:PRO_000023560 , Pride:P21369 , Protein Model Portal:P21369 , RefSeq:NP_416282 , RegulonDB:EG11135 , SMR:P21369 , String:511145.b1768 , UniProt:P21369
|Biological Process:||GO:0008152 - metabolic process
GO:0019363 - pyridine nucleotide biosynthetic process [UniProtGOA11a]
|Molecular Function:||GO:0008936 - nicotinamidase activity
GO:0003824 - catalytic activity [GOA01a]
GO:0016787 - hydrolase activity [UniProtGOA11a]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||metabolism → biosynthesis of building blocks → cofactors, small molecule carriers → nicotinamide adenine dinucleotide|
Enzymatic reaction of: nicotinamidase
Synonyms: nicotinamide amidohydrolase, NAm amidohydrolase, NAm deamidase, nicotinamide deamidase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: NAD salvage pathway I
EC Number: 3.5.1.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Reversibility of this reaction is unspecified.
Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.
The pncA-encoded nicotinamidase also has pyrazinamidase activity. Pyrazinamide is an antibiotic with a structure similar to nicotinamide [Frothingham96]
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
7/20/1999 (pkarp) Merged genes G188/pncA and EG11135/b1768
10/20/97 Gene b1768 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11135; confirmed by SwissProt match.
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Frothingham96: Frothingham R, Meeker-O'Connell WA, Talbot EA, George JW, Kreuzer KN (1996). "Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA." Antimicrob Agents Chemother 1996;40(6);1426-31. PMID: 8726014
Pardee71: Pardee AB, Benz EJ, St Peter DA, Krieger JN, Meuth M, Trieshmann HW (1971). "Hyperproduction and purification of nicotinamide deamidase, a microconstitutive enzyme of Escherichia coli." J Biol Chem 1971;246(22);6792-6. PMID: 4399474
Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293
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