Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: sarcosine-dimethylglycine methyltransferase [multifunctional]

Gene: AF216283 Accession Number: G-6503 (MetaCyc)

Species: Actinopolyspora halophila

Summary:
The sarcosine-dimethylglycine methyltransferase (SDMT) from Actinopolyspora halophila was purified from its native host, and found to poccess two activities: in addition to sarcosine, it can also accept dimethylglycine as an acceptor, resulting in dimethylglycine-betaine methyltransferase (DBMT) activity.

The gene encoding this protein was identified and sequenced, and found to encode a fusion protein, the N-terminal of which is similar to the gene encoding glycine-sarcosine methyltransferase (GSMT) in Halorhodospira halochloris. Thus, the authors speculate that this enzyme is most likely a proteolytic processing product.

When the complete gene was cloned and expressed in Escherichia coli, there was little activity, and most of the protein was found in the form of insoluble aggregates. However, when truncated versions of the gene were designed, encoding the GSMT and SDMT moieties separately, the SDMT was successfully expressed in soluble active form [Highland75].

Molecular Weight of Polypeptide: 32 kD (experimental) [Highland75 ]

Unification Links: ModBase:Q9KJ20 , Protein Model Portal:Q9KJ20 , Swiss-Model:Q9KJ20 , UniProt:Q9KJ20

Relationship Links: Entrez-Nucleotide:PART-OF:AF216283 , InterPro:IN-FAMILY:IPR013216 , InterPro:IN-FAMILY:IPR014369 , InterPro:IN-FAMILY:IPR023143 , Panther:IN-FAMILY:PTHR16458 , Pfam:IN-FAMILY:PF08241 , Prosite:IN-FAMILY:PS51600

Gene-Reaction Schematic: ?

MultiFun Terms: metabolism carbon utilization amines


Enzymatic reaction of: sarcosine-dimethylglycine methyltransferase

S-adenosyl-L-methionine + sarcosine <=> S-adenosyl-L-homocysteine + dimethylglycine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: glycine betaine biosynthesis IV (from glycine)


Enzymatic reaction of: dimethylglycine-betaine methyltransferase

EC Number: 2.1.1.161

S-adenosyl-L-methionine + dimethylglycine <=> S-adenosyl-L-homocysteine + glycine betaine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: glycine betaine biosynthesis IV (from glycine)


References

Highland75: Highland JH, Howard GA (1975). "Assembly of ribosomal proteins L7, L10, L11, and L12, on the 50 S subunit of Escherichia coli." J Biol Chem 250(3);813-4. PMID: 1089653


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.