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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: sarcosine-dimethylglycine methyltransferase [multifunctional]

Gene: AF216282 Accession Number: G-6502 (MetaCyc)

Species: Halorhodospira halochloris

Summary:
The sarcosine-dimethylglycine methyltransferase (SDMT) of Halorhodospira halochloris was not purified from its native organism despite efforts to do so. However, the gene encoding it was found next to the gene encoding it's sister enzyme - the glycine-sarcosine methyltransferase (GSMT). The gene was cloned, and the enzyme was successfully expressed in Escherichia coli.

The enzyme poccesses two activities - both sarcosine and dimethylglycine are recognized as substrates, resulting in both SDMT and dimethylglycine-betaine methyltransferase (DBMT) activities. Combined with the cloned gene encoding GSMT from the same organism, the two enzymes enabled Escherichia coli to convert glycine to glycine betaine [Nyyssola00].

It should be noted that both SDMT and GSMT from H. halochloris have SDMT activity (acceptng sarcosine as a substrate), so that they overlap in that respect. However, only GSMT can accept glycine as a substrate, and only SDMT can accept dimethylglycine as a substrate.

Gene Citations: [Highland75]

Molecular Weight of Polypeptide: 32.227 kD (from nucleotide sequence)

Unification Links: ModBase:Q9KJ21 , Protein Model Portal:Q9KJ21 , Swiss-Model:Q9KJ21 , UniProt:Q9KJ21

Relationship Links: Entrez-Nucleotide:PART-OF:AF216282 , InterPro:IN-FAMILY:IPR013216 , InterPro:IN-FAMILY:IPR023143 , Pfam:IN-FAMILY:PF08241

Gene-Reaction Schematic: ?

MultiFun Terms: metabolism carbon utilization amines


Enzymatic reaction of: sarcosine-dimethylglycine methyltransferase

S-adenosyl-L-methionine + sarcosine <=> S-adenosyl-L-homocysteine + dimethylglycine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: glycine betaine biosynthesis IV (from glycine)


Enzymatic reaction of: dimethylglycine-betaine methyltransferase

EC Number: 2.1.1.161

S-adenosyl-L-methionine + dimethylglycine <=> S-adenosyl-L-homocysteine + glycine betaine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: glycine betaine biosynthesis IV (from glycine)

Kinetic Parameters:

Substrate
Km (μM)
Citations
S-adenosyl-L-methionine
160.0
[Nyyssola01]
dimethylglycine
4900.0
[Nyyssola01]

pH(opt): 7.6 [Nyyssola01]


References

Highland75: Highland JH, Howard GA (1975). "Assembly of ribosomal proteins L7, L10, L11, and L12, on the 50 S subunit of Escherichia coli." J Biol Chem 250(3);813-4. PMID: 1089653

Nyyssola00: Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T (2000). "Extreme halophiles synthesize betaine from glycine by methylation." J Biol Chem 2000;275(29);22196-201. PMID: 10896953

Nyyssola01: Nyyssola A, Reinikainen T, Leisola M (2001). "Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase." Appl Environ Microbiol 67(5);2044-50. PMID: 11319079


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc13.