|Gene:||treS||Accession Number: G-4681 (MetaCyc)|
Species: Thermus aquaticus
A thermostable trehalose synthase was isolated from T. aquaticus, that was stable from pH 5.5.to 9.5 and up to 80oC for 60 minutes. The yield of trehalose from maltose was independent of the substrate concentration, and was higher at lower temperatures. Maximum yield reached 82% at 30-40oC [Nishimoto96].
Gene Citations: [Tsusaki97]
Molecular Weight of Polypeptide: 105 kD (experimental) [Nishimoto96 ]
pI: 4.6 [Nishimoto96]
|MultiFun Terms:||metabolism → carbon utilization → carbon compounds|
Enzymatic reaction of: trehalose synthase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
This reaction is reversible.
In Pathways: trehalose biosynthesis IV
T(opt): 65 °C [Nishimoto96]
pH(opt): 6.5 [Nishimoto96]
Nishimoto96: Nishimoto, T., Nakada, T., Chaen, H., Fukuda, S., Sugimoto, T., Kurimoto, M., Tsujisaka, Y. (1996). "Purification and characterization of a thermostable trehalose synthase from Thermus aquaticus." Biosci. Biotech. Biochem. 60(5):835-839.
Tsusaki97: Tsusaki K, Nishimoto T, Nakada T, Kubota M, Chaen H, Fukuda S, Sugimoto T, Kurimoto M (1997). "Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923." Biochim Biophys Acta 1334(1);28-32. PMID: 9042362
©2015 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493