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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: trehalose synthase

Gene: treS Accession Number: G-4681 (MetaCyc)

Species: Thermus aquaticus

Summary:
A thermostable trehalose synthase was isolated from T. aquaticus, that was stable from pH 5.5.to 9.5 and up to 80oC for 60 minutes. The yield of trehalose from maltose was independent of the substrate concentration, and was higher at lower temperatures. Maximum yield reached 82% at 30-40oC [Nishimoto96].

Gene Citations: [Tsusaki97]

Molecular Weight of Polypeptide: 105 kD (experimental) [Nishimoto96 ]

pI: 4.6 [Nishimoto96]

Relationship Links: CAZy:IN-FAMILY:GH13 , Entrez-Nucleotide:PART-OF:D86216

Gene-Reaction Schematic: ?

MultiFun Terms: metabolism carbon utilization carbon compounds


Enzymatic reaction of: trehalose synthase

EC Number: 5.4.99.16

maltose <=> α,α-trehalose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: trehalose biosynthesis IV

Inhibitors (Unknown Mechanism): Cu2+ [Nishimoto96] , Hg2+ [Nishimoto96] , Zn2+ [Nishimoto96] , tris [Nishimoto96]

T(opt): 65 °C [Nishimoto96]

pH(opt): 6.5 [Nishimoto96]


References

Nishimoto96: Nishimoto, T., Nakada, T., Chaen, H., Fukuda, S., Sugimoto, T., Kurimoto, M., Tsujisaka, Y. (1996). "Purification and characterization of a thermostable trehalose synthase from Thermus aquaticus." Biosci. Biotech. Biochem. 60(5):835-839.

Tsusaki97: Tsusaki K, Nishimoto T, Nakada T, Kubota M, Chaen H, Fukuda S, Sugimoto T, Kurimoto M (1997). "Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923." Biochim Biophys Acta 1334(1);28-32. PMID: 9042362


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc12.