Species: Arthrobacter nicotianae FI1612
The bacterium Arthrobacter nicotianae FI1612 was isolated in an enrichment culture grown with indole-3-carboxylate as the sole carbon source. This strain demonstrated a high level of indole-3-carboxylate decarboxylase activity. Enzyme activity was strongly induced by adding indole-3-carboxylate to the medium.
The enzyme was purified, and found to be sensitive to oxygen. It did not require any cofactors, and could also catalyze the reverse reaction [Yoshida02a].
Enzymatic reaction of: indole-3-carboxylate decarboxylase
EC Number: 126.96.36.199indole-3-carboxylate + H+ ⇄ indole + CO2
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.
This reaction is reversible. [Yoshida02a]Alternative Substrates for indole: 2-methylindole [Yoshida02a] Inhibitors (Other): N-ethylmaleimide [Yoshida02a], o-phenanthroline [Yoshida02a], phenylhydrazine [Yoshida02a]Kinetic Parameters:
T(opt): 50 °C [Yoshida02a]
pH(opt): 7 [Yoshida02a]
Yoshida02a: Yoshida T, Fujita K, Nagasawa T (2002). "Novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation." Biosci Biotechnol Biochem 66(11);2388-94. PMID: 12506977
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