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Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: indole-3-carboxylate decarboxylase

Species: Arthrobacter nicotianae FI1612

Summary:
The bacterium Arthrobacter nicotianae FI1612 was isolated in an enrichment culture grown with indole-3-carboxylate as the sole carbon source. This strain demonstrated a high level of indole-3-carboxylate decarboxylase activity. Enzyme activity was strongly induced by adding indole-3-carboxylate to the medium.

The enzyme was purified, and found to be sensitive to oxygen. It did not require any cofactors, and could also catalyze the reverse reaction [Yoshida02].

Gene-Reaction Schematic: ?

Credits:
Created 13-Apr-2011 by Caspi R , SRI International


Enzymatic reaction of: indole-3-carboxylate decarboxylase

EC Number: 4.1.1.92

indole-3-carboxylate + H+ <=> indole + CO2

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Yoshida02]

Alternative Substrates for indole: 2-methylindole [Yoshida02 ]

Inhibitors (Other): N-ethylmaleimide [Yoshida02] , o-phenanthroline [Yoshida02] , phenylhydrazine [Yoshida02]

Kinetic Parameters:

Substrate
Km (μM)
Citations
indole
720.0
[Yoshida02]
indole-3-carboxylate
630.0
[Yoshida02]

T(opt): 50 °C [Yoshida02]

pH(opt): 7 [Yoshida02]


References

Yoshida02: Yoshida T, Fujita K, Nagasawa T (2002). "Novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation." Biosci Biotechnol Biochem 66(11);2388-94. PMID: 12506977


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13A.