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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: oleate 12-hydroxylase

Gene: FAH12 Accession Number: G-11805 (MetaCyc)

Synonyms: oleate 12-hydroxylase

Species: Ricinus communis

Summary:
A protein having oleoyl-12-hydroxylase activity was purified and characterized from microsomal fraction of immature castor bean. The actual substrate 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine (2-oleoyl-PC) was used for enzyme characterization, as an improvement over the previous use of oleoyl-CoA as substrate. This enzyme catalyzes the formation of the product ricinoleate. The enzyme fraction was found to peak in late anthesis stage of castor [Lin96].

Molecular Weight of Polypeptide: 44.3 kD (from nucleotide sequence), 44.3 kD (experimental) [Lin96 ]

Unification Links: Entrez-Nucleotide:EU523112 , Entrez:ACD39348 , UniProt:D2CPE1

Relationship Links: InterPro:IN-FAMILY:IPR005804 , InterPro:IN-FAMILY:IPR021863 , Pfam:IN-FAMILY:PF00487 , Pfam:IN-FAMILY:PF11960

Gene-Reaction Schematic: ?

Credits:
Created 17-Feb-2010 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: oleate 12-hydroxylase

EC Number: 1.14.13.-

(11Z)-eicos-11-enoyl-CoA + NADH + oxygen + H+ <=> lesqueroloyl-CoA + NAD+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: hydroxylated fatty acid biosynthesis (plants)


Enzymatic reaction of: oleate 12-hydroxylase

EC Number: 1.14.13.-

oleoyl-CoA + NADH + oxygen + H+ <=> ricinoleyl-CoA + NAD+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: hydroxylated fatty acid biosynthesis (plants)


Enzymatic reaction of: oleate 12-hydroxylase

oleate + NADH + oxygen + H+ <=> ricinoleate + NAD+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: ricinoleate biosynthesis


Enzymatic reaction of: oleate 12-hydroxylase

(11Z)-eicos-11-enoate + H2O <=> lesquerolate + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


References

Lin96: Lin JT, McKeon TA, Goodrich-Tanrikulu M, Stafford AE (1996). "Characterization of oleoyl-12-hydroxylase in castor microsomes using the putative substrate, 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine." Lipids 31(6);571-7. PMID: 8784737


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.