MetaCyc Enzyme: N6,N6,O-tridemethylpuromycin N-acetyltransferase

Gene: pac Accession Number: G-10676 (MetaCyc)

Species: Streptomyces alboniger

The subunit structure of this enzyme has not been reported.

The apparent molecular mass was determined by gel filtration chromatography [Vara85].

Gene Citations: [Lacalle89, Vara88, Vara85a]

Molecular Weight of Polypeptide: 21.496 kD (from nucleotide sequence), 23.0 kD (experimental) [Vara85 ]

Unification Links: Protein Model Portal:P13249 , UniProt:P13249

Relationship Links: Entrez-Nucleotide:PART-OF:M25346 , InterPro:IN-FAMILY:IPR000182 , InterPro:IN-FAMILY:IPR016181 , Pfam:IN-FAMILY:PF00583 , Prosite:IN-FAMILY:PS51186

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 14-May-2008 by Fulcher CA , SRI International

Enzymatic reaction of: N6,N6,O-tridemethylpuromycin N-acetyltransferase

Synonyms: puromycin N-acetyltransferase

N6,N6,O-tridemethylpuromycin + acetyl-CoA <=> N-acetyl-N6,N6,O-tridemethylpuromycin + coenzyme A + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: puromycin biosynthesis

The enzyme was partially purified from cell extracts of Streptomyces alboniger and its N-acetyltransferase activity was assayed using puromycin, O-demethylpuromycin and chryscandin as substrates [Vara85].

The recombinant enzyme was cloned and expressed in Escherichia coli and puromycin N-acetyltransferase activity was demonstrated [Vara85a].


Lacalle89: Lacalle RA, Pulido D, Vara J, Zalacain M, Jimenez A (1989). "Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger." Gene 79(2);375-80. PMID: 2676728

Vara85: Vara J, Perez-Gonzalez JA, Jimenez A (1985). "Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase." Biochemistry 24(27);8074-81. PMID: 4092057

Vara85a: Vara J, Malpartida F, Hopwood DA, Jimenez A (1985). "Cloning and expression of a puromycin N-acetyl transferase gene from Streptomyces alboniger in Streptomyces lividans and Escherichia coli." Gene 33(2);197-206. PMID: 3888783

Vara88: Vara JA, Pulido D, Lacalle RA, Jimenez A (1988). "Two genes in Streptomyces alboniger puromycin biosynthesis pathway are closely linked." Gene 69(1);135-40. PMID: 3066696

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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