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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: N6,N6,O-tridemethylpuromycin N-acetyltransferase

Gene: pac Accession Number: G-10676 (MetaCyc)

Species: Streptomyces alboniger

Summary:
The subunit structure of this enzyme has not been reported.

The apparent molecular mass was determined by gel filtration chromatography [Vara85].

Gene Citations: [Lacalle89, Vara88a, Vara85a]

Molecular Weight of Polypeptide: 21.496 kD (from nucleotide sequence), 23.0 kD (experimental) [Vara85 ]

Unification Links: Protein Model Portal:P13249 , UniProt:P13249

Relationship Links: Entrez-Nucleotide:PART-OF:M25346 , InterPro:IN-FAMILY:IPR000182 , InterPro:IN-FAMILY:IPR016181 , Pfam:IN-FAMILY:PF00583 , Prosite:IN-FAMILY:PS51186

Gene-Reaction Schematic: ?

Credits:
Created 14-May-2008 by Fulcher CA , SRI International


Enzymatic reaction of: N6,N6,O-tridemethylpuromycin N-acetyltransferase

Synonyms: puromycin N-acetyltransferase

N6,N6,O-tridemethylpuromycin + acetyl-CoA <=> N-acetyl-N6,N6,O-tridemethylpuromycin + coenzyme A + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: puromycin biosynthesis

Summary:
The enzyme was partially purified from cell extracts of Streptomyces alboniger and its N-acetyltransferase activity was assayed using puromycin, O-demethylpuromycin and chryscandin as substrates [Vara85].

The recombinant enzyme was cloned and expressed in Escherichia coli and puromycin N-acetyltransferase activity was demonstrated [Vara85a].


References

Lacalle89: Lacalle RA, Pulido D, Vara J, Zalacain M, Jimenez A (1989). "Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger." Gene 79(2);375-80. PMID: 2676728

Vara85: Vara J, Perez-Gonzalez JA, Jimenez A (1985). "Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase." Biochemistry 24(27);8074-81. PMID: 4092057

Vara85a: Vara J, Malpartida F, Hopwood DA, Jimenez A (1985). "Cloning and expression of a puromycin N-acetyl transferase gene from Streptomyces alboniger in Streptomyces lividans and Escherichia coli." Gene 33(2);197-206. PMID: 3888783

Vara88a: Vara JA, Pulido D, Lacalle RA, Jimenez A (1988). "Two genes in Streptomyces alboniger puromycin biosynthesis pathway are closely linked." Gene 69(1);135-40. PMID: 3066696


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc14.