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MetaCyc Enzyme: holo-DhbF protein

Gene: dhbF Accession Number: G-10624 (MetaCyc)

Species: Bacillus subtilis

Component of: bacillibactin synthase multienzyme complex

Alternative forms of holo-DhbF protein: apo-DhbF protein (summary available)

Summary:
This protein is a very large dimodular enzyme, that contains 7 domains, responsible for glycine binding, adenylation, and condensation with 2,3-dihydroxybenzoate (DHB), L-threonine binding, adenylation, and condensation with 2,3-dihydroxybenzoylglycine, and finally, a Te domain, responsible of condensation of three DHB-glycine-threonine into a single bacillibactin molecule [May01].

The apo enzyme is activated by binding two molecules of 4'-phosphopantetheine, in a reaction catalyzed by the sfp-encoded 4'-phosphopantetheinyl transferase [May01]. The activated enzyme is referred to as holo-DhbF protein.

A null mutant lacking DhbF was constructed, and failed to produce bacillibactin [May01].

Relationship Links: Entrez-Nucleotide:PART-OF:AF184977

Gene-Reaction Schematic: ?

Credits:
Created 29-Apr-2008 by Caspi R , SRI International


Enzymatic reaction of: L-threonine-AMP ligase (holo-DhbF protein)

L-threonine + ATP <=> L-threonyl-AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Enzymatic reaction of: glycine-AMP ligase (holo-DhbF protein)

glycine + ATP <=> glycyl-AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Subunit of: bacillibactin synthase multienzyme complex

Species: Bacillus subtilis

Subunit composition of bacillibactin synthase multienzyme complex = [([DhbB]5)][(DhbE)2][DhbF]
         holo-DhbB protein = ([DhbB]5) (summary available)
                 apo-DhbB pentamer = (DhbB)5
                         apo DhbB monomer = DhbB
         2,3-dihydroxybenzoate-AMP ligase = (DhbE)2 (summary available)
                 DhbE monomer = DhbE
         holo-DhbF protein = DhbF (summary available)

Credits:
Created 29-Apr-2008 by Caspi R , SRI International


Enzymatic reaction of: bacillibactin synthase

3 2,3-dihydroxybenzoate + 3 glycine + 3 L-threonine + 6 ATP <=> bacillibactin + 6 AMP + 6 diphosphate + 3 H+ + 3 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: bacillibactin biosynthesis


References

May01: May JJ, Wendrich TM, Marahiel MA (2001). "The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin." J Biol Chem 276(10);7209-17. PMID: 11112781


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Jan 31, 2015, BIOCYC14B.