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MetaCyc Enzyme: acetaldehyde dehydrogenase (acylating)

Gene: dmpF Accession Number: G-9793 (MetaCyc)

Species: Pseudomonas sp. CF600

Component of: bifunctional aldolase-dehydrogenase (summary available)

Summary:
The final two steps of the meta-cleavage pathway in Pseudomonas sp. CF600 involve the conversion of 4-hydroxy-2-oxopentanoate to pyruvate and acetyl-CoA by the enzymes 4-hydroxy-2-oxovalerate aldolase and acetaldehyde dehydrogenase (acylating).

Biochemical studies demonstrated that these two enzymes comprise a bifunctional aldolase-dehydrogenase heterodimer, and suggest that the product of the aldolase reaction, acetaldehyde, is transferred to the dehydrogenase active site via a channeling mechanism. This minimizes the risk to the cells posed by the toxic acetaldehyde [Powlowski93].

Gene Citations: [Shingler92]

Molecular Weight of Polypeptide: 32.685 kD (from nucleotide sequence), 34.5 kD (experimental) [Powlowski93 ]

Unification Links: Protein Model Portal:Q52060 , SMR:Q52060 , UniProt:Q52060

Relationship Links: Entrez-Nucleotide:RELATED-TO:X60835 , InterPro:IN-FAMILY:IPR000534 , InterPro:IN-FAMILY:IPR003361 , InterPro:IN-FAMILY:IPR015426 , InterPro:IN-FAMILY:IPR016040 , Panther:IN-FAMILY:PTHR21123 , PDB:Structure:1NVM , Pfam:IN-FAMILY:PF01118 , Pfam:IN-FAMILY:PF09290 , Smart:IN-FAMILY:SM00859

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 07-Dec-2006 by Caspi R , SRI International


Enzymatic reaction of: acetaldehyde dehydrogenase (acylating) (acetaldehyde dehydrogenase (acylating))

EC Number: 1.2.1.10

acetaldehyde + coenzyme A + NAD+ <=> acetyl-CoA + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

Alternative Substrates for acetaldehyde: propanal [Powlowski93 ]

In Pathways: meta cleavage pathway of aromatic compounds , catechol degradation II (meta-cleavage pathway) , catechol degradation I (meta-cleavage pathway) , 2-oxopentenoate degradation

Summary:
Activity of the enzyme was as follows (in nanomoles per minute):

acetaldehyde: 58.5

propoinaldehyde: 35.6

butyraldehyde: 14.9

isobutyraldehyde: 10.5

formaldehyde: <2

pH(opt): 8.5 [Powlowski93]


Subunit of: bifunctional aldolase-dehydrogenase

Species: Pseudomonas sp. CF600

Subunit composition of bifunctional aldolase-dehydrogenase = [DmpG]2[DmpF]2
         4-hydroxy-2-oxovalerate aldolase = DmpG (summary available)
         acetaldehyde dehydrogenase (acylating) = DmpF (summary available)

Summary:
The final two steps of the meta-cleavage pathway in Pseudomonas sp. CF600 involve the conversion of 4-hydroxy-2-oxopentanoate to pyruvate and acetyl-CoA by the enzymes 4-hydroxy-2-oxovalerate aldolase and acetaldehyde dehydrogenase (acylating).

Biochemical studies demonstrated that these two enzymes comprise a bifunctional aldolase-dehydrogenase heterodimer, and suggest that the product of the aldolase reaction, acetaldehyde, is transferred to the dehydrogenase active site via a channeling mechanism. This minimizes the risk to the cells posed by the toxic acetaldehyde.

The heterodimer has been crystallized and its structure determined. The assymetric unit contains four DmpFG heterodimers [Manjasetty01].

Molecular Weight: 148 kD (experimental) [Powlowski93]

Credits:
Created 07-Dec-2006 by Caspi R , SRI International


References

Manjasetty01: Manjasetty BA, Croteau N, Powlowski J, Vrielink A (2001). "Crystallization and preliminary X-ray analysis of dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase--aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600." Acta Crystallogr D Biol Crystallogr 57(Pt 4);582-5. PMID: 11264589

Powlowski93: Powlowski J, Sahlman L, Shingler V (1993). "Purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600." J Bacteriol 175(2);377-85. PMID: 8419288

Shingler92: Shingler V, Powlowski J, Marklund U (1992). "Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600." J Bacteriol 174(3);711-24. PMID: 1732207


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Sep 1, 2015, BIOCYC14B.