Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: tartrate dehydrogenase

Species: Pseudomonas putida

Subunit composition of tartrate dehydrogenase = [tartrate dehydrogenase subunit]4

Summary:
tartrate dehydrogenase has been purified and crystallized from Pseudomonas putida. The enzyme catalyzes the reversible, NAD-linked oxidation of meso-tartrate and of L-tartrate to 2-hydroxy-3-oxosuccinate. D-tartrate was not a substrate. (S)-malate and (R)-malate were inactive as either substrates or inhibitors, suggesting this enzyme os different from another Pseudomonas putida enzyme that is able to catalyze this reaction (see tartrate dehydrogenase/decarboxylase).

The enzyme requires Mn2+ ions and one of several monovalent cations for activity. Both qualitative and quantitative differences were found in the requirement for monovalent cation, depending on the nature of the substrate [Kohn68].

Molecular Weight of Polypeptide: 36.8 kD (experimental) [Kohn68 ]

Molecular Weight of Multimer: 145 kD (experimental) [Kohn68]

Gene-Reaction Schematic: ?

Credits:
Created 31-Jan-2007 by Caspi R , SRI International


Enzymatic reaction of: tartrate dehydrogenase

EC Number: 1.1.1.93

L-tartrate + NAD+ <=> 2-hydroxy-3-oxosuccinate + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Activators (Allosteric): Mn2+ [Kohn68] , K+ [Kohn68] , Rb+ [Kohn68]

Inhibitors (Competitive): dihydroxyfumarate [Kohn68]

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-tartrate
55000.0
[Kohn68]
NAD+
90.0
[Kohn68]

pH(opt): 8.5 [Kohn68]


References

Kohn68: Kohn LD, Packman PM, Allen RH, Jakoby WB (1968). "Tartaric acid metabolism. V. Crystalline tartrate dehydrogenase." J Biol Chem 243(10);2479-85. PMID: 4297261


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, biocyc14.