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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: farnesyl diphosphate synthase

Gene: GGPPS Accession Number: GGPS1-C (MetaCyc)

Synonyms: GGPP synthase, GPP synthase, FPP synthase

Species: Capsicum annuum

Subunit composition of farnesyl diphosphate synthase = [GGPPS]2
         prenyltransferase subunit = GGPPS

Summary:
The enzyme was isolated by affinity chromatography and purified to homogeneity from Capsicum chromoplasts [Dogbo87]. It is a homodimeric enzyme. In chromoplasts, this protein behaves as an integral component of the phytoene synthase complex [Dogbo87] involved in carotenoid biosynthesis. The enzyme is devoid of isopentenyl pyrophosphate isomerase activity.

Citations: [Badillo95]

Gene Citations: [Badillo95]

Locations: chromoplast

Molecular Weight of Polypeptide: 37 kD (experimental) [Dogbo87 ]

Molecular Weight of Multimer: 74 kD (experimental) [Dogbo87]

Unification Links: Entrez:CAA56554 , Protein Model Portal:P80042 , SMR:P80042 , UniProt:P80042

Relationship Links: Entrez-Nucleotide:PART-OF:X80267 , InterPro:IN-FAMILY:IPR000092 , InterPro:IN-FAMILY:IPR008949 , InterPro:IN-FAMILY:IPR017446 , Panther:IN-FAMILY:PTHR12001 , Pfam:IN-FAMILY:PF00348 , Prosite:IN-FAMILY:PS00444 , Prosite:IN-FAMILY:PS00723

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0009509 - chromoplast [Dogbo87]
GO:0009536 - plastid [Kuntz92]


Enzymatic reaction of: geranyl pyrophosphate synthase (farnesyl diphosphate synthase)

EC Number: 2.5.1.1

dimethylallyl diphosphate + isopentenyl diphosphate <=> geranyl diphosphate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)

Kinetic Parameters:

Substrate
Km (μM)
Citations
dimethylallyl diphosphate
0.95
[Dogbo87]
isopentenyl diphosphate
3.0
[Dogbo87]


Enzymatic reaction of: ω,E,E-farnesyl diphosphate synthase

EC Number: 2.5.1.10

geranyl diphosphate + isopentenyl diphosphate <=> (2E,6E)-farnesyl diphosphate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)

Kinetic Parameters:

Substrate
Km (μM)
Citations
geranyl diphosphate
1.0
isopentenyl diphosphate
3.0
[Dogbo87]


Enzymatic reaction of: geranylgeranyl pyrophosphate synthase (farnesyl diphosphate synthase)

EC Number: 2.5.1.29

(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate <=> geranylgeranyl diphosphate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) , geranylgeranyl diphosphate biosynthesis

Kinetic Parameters:

Substrate
Km (μM)
Citations
(2E,6E)-farnesyl diphosphate
1.5
[Dogbo87]
isopentenyl diphosphate
3.0
[Dogbo87]


Sequence Features

Feature Class Location Citations Comment
Transit-Peptide unspecified
[Kuntz92]
Putative plastid (chromoplast and presumably chloroplast) targeting sequence at N-terminus of protein (60 a.a). Cleavage would occur somewhere between position 57 and 61 [Kuntz92].


References

Badillo95: Badillo A, Steppuhn J, Deruere J, Camara B, Kuntz M (1995). "Structure of a functional geranylgeranyl pyrophosphate synthase gene from Capsicum annuum." Plant Mol Biol 27(2);425-8. PMID: 7888631

Dogbo87: Dogbo, O., Camara, B. (1987). "Purification of isopentenyl pyrophosphate isomerase and geranylgeranyl pyrophosphate synthase from Capsicum chromoplasts by affinity chromatography." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 920(2):140-148.

Kuntz92: Kuntz M, Romer S, Suire C, Hugueney P, Weil JH, Schantz R, Camara B (1992). "Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening." Plant J 2(1);25-34. PMID: 1303794


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc14.