MetaCyc Enzyme: D-tagatose-6-phosphate kinase

Gene: lacC Accession Number: G-1921 (MetaCyc)

Synonyms: subunit of D-tagatose-6-phosphate kinase

Species: Staphylococcus aureus

Subunit composition of D-tagatose-6-phosphate kinase = [LacC]2
         D-tagatose-6-phosphate kinase subunit = LacC

Citations: [Bissett80, Rosey91]

Gene Citations: [Rosey91]

Locations: cytosol

Molecular Weight of Polypeptide: 34.0 kD (from nucleotide sequence), 52.0 kD (experimental)

Molecular Weight of Multimer: 82 kD (experimental)

Unification Links: ModBase:P11099 , Protein Model Portal:P0A0B8 , SMR:P0A0B8 , String:196620.MW2119 , Swiss-Model:P11099 , UniProt:P0A0B8

Relationship Links: InterPro:IN-FAMILY:IPR002173 , InterPro:IN-FAMILY:IPR005926 , InterPro:IN-FAMILY:IPR011611 , InterPro:IN-FAMILY:IPR017583 , InterPro:IN-FAMILY:IPR029056 , Pfam:IN-FAMILY:PF00294 , Prosite:IN-FAMILY:PS00583 , Prosite:IN-FAMILY:PS00584

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005829 - cytosol

Enzymatic reaction of: D-tagatose-6-phosphate kinase

Synonyms: tagatose-6-phosphate isomerase

EC Number:

D-tagatofuranose 6-phosphate + ATP <=> D-tagatofuranose 1,6-bisphosphate + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for ATP: dTTP [Bissett80 ] , CTP [Bissett80 ] , ITP [Bissett80 ] , UTP [Bissett80 ] , GTP [Bissett80 ]

Alternative Substrates for D-tagatofuranose 6-phosphate: β-D-fructofuranose 6-phosphate [Bissett80 ]

In Pathways: lactose and galactose degradation I

D-Tagatose-6-phosphate kinase catalyzes the ATP-dependent phosphorylation of D-tagatose-6-phosphate, a reaction in the tagatose 6-phosphate pathway of lactose and galactose degradation in Staphylococcus aureus [Bissett80].

D-Tagatose-6-phosphate kinase was partially purified and characterized [Bissett80]. Data on kinetics, pH optimum, enzyme stability, and inducibility are presented and discussed [Bissett80].

D-Tagatose-6-phosphate kinase can catalyze the phosphorylation of tagatose-6-phosphate and fructose-6-phosphate. Although the VMAX was the same, the apparent KM for tagatose-6-phosphate and fructose-6-phosphate was significantly different,16 microM and 150 mM, respectively [Bissett80].

D-Tagatose-6-phosphate kinase can use the following phosphoryl donors (listed in decreasing rates at saturating concentrations): GTP, UTP, ITP, ATP, CTP, TTP. The rates and KM values are reported [Bissett80].

D-Tagatose-6-phosphate kinase was induced by growth of Staphylococcus aureus cells on lactose and D-galactose [Bissett80].

D-Tagatose-6-phosphate kinase belongs to the carbohydrate pfkB kinase family (Swiss-Prot).

Cofactors or Prosthetic Groups: Mg2+

Activators (Unknown Mechanism): Cs+ [Bissett80] , Rb+ [Bissett80] , ammonium [Bissett80] , K+ [Bissett80]

Inhibitors (Unknown Mechanism): Li+ [Bissett80] , Na+ [Bissett80]


Bissett80: Bissett DL, Anderson RL (1980). "Lactose and D-galactose metabolism in Staphylococcus aureus. III. Purification and properties of D-tagatose-6-phosphate kinase." J Biol Chem 255(18);8745-9. PMID: 6251066

Rosey91: Rosey EL, Oskouian B, Stewart GC (1991). "Lactose metabolism by Staphylococcus aureus: characterization of lacABCD, the structural genes of the tagatose 6-phosphate pathway." J Bacteriol 173(19);5992-8. PMID: 1655695

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, biocyc14.