|Gene:||lacC||Accession Number: G-1921 (MetaCyc)|
Synonyms: subunit of D-tagatose-6-phosphate kinase
Species: Staphylococcus aureus
Subunit composition of
D-tagatose-6-phosphate kinase = [LacC]2
D-tagatose-6-phosphate kinase subunit = LacC
Gene Citations: [Rosey91]
Molecular Weight of Polypeptide: 34.0 kD (from nucleotide sequence), 52.0 kD (experimental)
Molecular Weight of Multimer: 82 kD (experimental)
Relationship Links: InterPro:IN-FAMILY:IPR002173 , InterPro:IN-FAMILY:IPR005926 , InterPro:IN-FAMILY:IPR011611 , InterPro:IN-FAMILY:IPR017583 , InterPro:IN-FAMILY:IPR029056 , Pfam:IN-FAMILY:PF00294 , Prosite:IN-FAMILY:PS00583 , Prosite:IN-FAMILY:PS00584
|Cellular Component:||GO:0005829 - cytosol|
Enzymatic reaction of: D-tagatose-6-phosphate kinase
Synonyms: tagatose-6-phosphate isomerase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: lactose and galactose degradation I
D-Tagatose-6-phosphate kinase catalyzes the ATP-dependent phosphorylation of D-tagatose-6-phosphate, a reaction in the tagatose 6-phosphate pathway of lactose and galactose degradation in Staphylococcus aureus [Bissett80].
D-Tagatose-6-phosphate kinase can catalyze the phosphorylation of tagatose-6-phosphate and fructose-6-phosphate. Although the VMAX was the same, the apparent KM for tagatose-6-phosphate and fructose-6-phosphate was significantly different,16 microM and 150 mM, respectively [Bissett80].
D-Tagatose-6-phosphate kinase can use the following phosphoryl donors (listed in decreasing rates at saturating concentrations): GTP, UTP, ITP, ATP, CTP, TTP. The rates and KM values are reported [Bissett80].
D-Tagatose-6-phosphate kinase was induced by growth of Staphylococcus aureus cells on lactose and D-galactose [Bissett80].
D-Tagatose-6-phosphate kinase belongs to the carbohydrate pfkB kinase family (Swiss-Prot).
Cofactors or Prosthetic Groups: Mg2+
Bissett80: Bissett DL, Anderson RL (1980). "Lactose and D-galactose metabolism in Staphylococcus aureus. III. Purification and properties of D-tagatose-6-phosphate kinase." J Biol Chem 255(18);8745-9. PMID: 6251066
Rosey91: Rosey EL, Oskouian B, Stewart GC (1991). "Lactose metabolism by Staphylococcus aureus: characterization of lacABCD, the structural genes of the tagatose 6-phosphate pathway." J Bacteriol 173(19);5992-8. PMID: 1655695
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