Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: alanine transaminase

Gene: AOAT1 Accession Number: AT1G23310 (MetaCyc)

Synonyms: GGAT1

Species: Arabidopsis thaliana col

Summary:
AOAT1 (GGAT1) is peroxisomal located. Knock-out plants have reduced activity of AOAT, GPAT (glutamate:pyruvate aminotransferase), AGAT (alanine:glyoxylate aminotransferase) and GGAT (glutamate:glyoxylate aminotransferase). The GGAT and AGAT activities were reduced most drastically. These indicate AOAT1 is primarily involved in photorespiration [Igarashi03]. Similary, AOAT2 (GGAT2), which is predicted to be located in the peroxisome, is likely involved in photorespiration.

In vitro assay of the recombinant proteins indicated that GGAT1 and GGAT2 have four aminotransferase activities, namely GGAT, AGAT, GPAT and AOAT. The two recombinant proteins exhibited very similar Km values towards amino acid substrates glutamate and alanine, as well as the oxoacid substrates glyoxylate, pyruvate and 2-oxoglutarate [Liepman03].

Map Position: [8,268,393 -> 8,271,922] (27.17 centisomes)

Unification Links: PhylomeDB:Q9LR30 , Pride:Q9LR30 , Protein Model Portal:Q9LR30 , SMR:Q9LR30 , String:3702.AT1G23310.1-P , TAIR:AT1G23310 , UniProt:Q9LR30

Relationship Links: InterPro:IN-FAMILY:IPR004839 , InterPro:IN-FAMILY:IPR015421 , InterPro:IN-FAMILY:IPR015422 , InterPro:IN-FAMILY:IPR015424 , Pfam:IN-FAMILY:PF00155 , Prints:IN-FAMILY:PR00753

Gene-Reaction Schematic: ?

Gene Class: UNCLASSIFIED


Enzymatic reaction of: alanine:glyoxylate aminotransferase (alanine transaminase)

Synonyms: Ala:glyoxylate aminotransferase

EC Number: 2.6.1.44

glyoxylate + L-alanine <=> glycine + pyruvate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.


Enzymatic reaction of: alanine:2-oxoglutarate aminotransferase (alanine transaminase)

Synonyms: alanine:2-ketoglutarate aminotransferase, Ala:2-oxoglutarate aminotransferase

EC Number: 2.6.1.2

2-oxoglutarate + L-alanine <=> L-glutamate + pyruvate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.


Enzymatic reaction of: glycine transaminase (alanine transaminase)

EC Number: 2.6.1.4

2-oxoglutarate + glycine <=> L-glutamate + glyoxylate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

In Pathways: photorespiration

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-glutamate
1970.0
[Liepman03]
glyoxylate
210.0
[Liepman03]


References

Igarashi03: Igarashi D, Miwa T, Seki M, Kobayashi M, Kato T, Tabata S, Shinozaki K, Ohsumi C (2003). "Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis." Plant J 33(6);975-87. PMID: 12631323

Liepman03: Liepman AH, Olsen LJ (2003). "Alanine aminotransferase homologs catalyze the glutamate:glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis." Plant Physiol 131(1);215-27. PMID: 12529529


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14A.