MetaCyc Enzyme: phospho-N-acetylmuramoyl-pentapeptide transferase

Gene: mraY Accession Numbers: EG10604 (MetaCyc), b0087, ECK0088

Synonyms: murX

Species: Escherichia coli K-12 substr. MG1655

Phospho-N-acetylmuramoyl-pentapeptide transferase catalyzes the first step of the lipid cycle reactions in the biosynthetic pathway of cell wall peptidoglycans [Ikeda91a].

Locations: inner membrane

Map Position: [96,002 -> 97,084]

Molecular Weight of Polypeptide: 39.875 kD (from nucleotide sequence)

pI: 9.73

Unification Links: ASAP:ABE-0000316 , CGSC:30466 , EchoBASE:EB0599 , EcoGene:EG10604 , EcoliWiki:b0087 , OU-Microarray:b0087 , PortEco:mraY , PR:PRO_000023283 , Protein Model Portal:P0A6W3 , RefSeq:NP_414629 , RegulonDB:EG10604 , SMR:P0A6W3 , String:511145.b0087 , UniProt:P0A6W3

Relationship Links: InterPro:IN-FAMILY:IPR000715 , InterPro:IN-FAMILY:IPR003524 , InterPro:IN-FAMILY:IPR018480 , Panther:IN-FAMILY:PTHR22926 , Pfam:IN-FAMILY:PF00953 , Pfam:IN-FAMILY:PF10555 , Prosite:IN-FAMILY:PS01347 , Prosite:IN-FAMILY:PS01348

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0007049 - cell cycle Inferred by computational analysis [UniProtGOA11a]
GO:0008360 - regulation of cell shape Inferred by computational analysis [UniProtGOA11a]
GO:0009252 - peptidoglycan biosynthetic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11a, GOA06]
GO:0051301 - cell division Inferred by computational analysis [UniProtGOA11a]
GO:0071555 - cell wall organization Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0008963 - phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01, GOA01a, Ikeda91a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0051992 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
cell structure murein
metabolism biosynthesis of macromolecules (cellular constituents) murein (peptidoglycan)

Imported from EcoCyc 02-Jun-2015 by Paley S , SRI International

Enzymatic reaction of: phospho-N-acetylmuramoyl-pentapeptide transferase

Synonyms: UDPMurAc(oyl-L-Ala-D-γ-Glu-L-Lys-D-Ala-D-Ala): undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase, UDPMurAc(oyl-L-Ala-D-γ-Glu-meso-DAP-D-Ala-D-Ala): undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase

UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + di-trans,octa-cis-undecaprenyl phosphate <=> undecaprenyldiphospho-N-acetylmuramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

Alternative Substrates [Comment 1]:

In Pathways: peptidoglycan biosynthesis I (meso-diaminopimelate containing)

Imported from EcoCyc 02-Jun-2015 by Paley S , SRI International

Inhibitors (Unknown Mechanism): tunicamycin [Ikeda91a]

Kinetic Parameters:

Km (μM)
67.0, 87.0
[Geis78, BRENDA14]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 19 -> 45
UniProt: Helical.
Transmembrane-Region 77 -> 90
UniProt: Helical.
Transmembrane-Region 97 -> 113
UniProt: Helical.
Transmembrane-Region 134 -> 156
UniProt: Helical.
Transmembrane-Region 174 -> 188
UniProt: Helical.
Transmembrane-Region 200 -> 220
UniProt: Helical.
Transmembrane-Region 239 -> 251
UniProt: Helical.
Transmembrane-Region 271 -> 284
UniProt: Helical.
Transmembrane-Region 288 -> 299
UniProt: Helical.
Transmembrane-Region 342 -> 357
UniProt: Helical.

10/20/97 Gene b0087 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10604; confirmed by SwissProt match.


BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014."

Geis78: Geis A, Plapp R (1978). "Phospho-N-acetylmuramoyl-pentapeptide-transferase of Escherichia coli K12. Properties of the membrane-bound and the extracted and partially purified enzyme." Biochim Biophys Acta 527(2);414-24. PMID: 215212

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Ikeda91a: Ikeda M, Wachi M, Jung HK, Ishino F, Matsuhashi M (1991). "The Escherichia coli mraY gene encoding UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase." J Bacteriol 1991;173(3);1021-6. PMID: 1846850

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, BIOCYC14B.