MetaCyc Enzyme: precorrin-5 (C1)-methyltransferase

Gene: cobF Accession Number: G-499 (MetaCyc)

Synonyms: precorrin 6A synthase

Species: Pseudomonas denitrificans

The Pseudomonas denitrificans enzyme precorrin-5 (C1)-methyltransferase catalyzes two reactions - the methylation of carbon C1 of precorrin-5, and its deacetylation, forming precorrin-6A. The cobF gene encoding the enzyme has been cloned and sequenced, and the enzyme, which requires S-adenosyl-L-methionine for activity, has been purified [Debussche93].

Gene Citations: [Crouzet90]

Molecular Weight of Polypeptide: 28.945 kD (from nucleotide sequence), 31 kD (experimental) [Debussche93 ]

Unification Links: ModBase:P21636 , Protein Model Portal:P21636 , Swiss-Model:P21636 , UniProt:P21636

Relationship Links: Entrez-Nucleotide:RELATED-TO:M59301 , InterPro:IN-FAMILY:IPR000878 , InterPro:IN-FAMILY:IPR012797 , InterPro:IN-FAMILY:IPR014776 , InterPro:IN-FAMILY:IPR014777 , Pfam:IN-FAMILY:PF00590

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 18-Jan-2001 by Pellegrini-Toole A , Marine Biological Laboratory
Revised 28-Jun-2007 by Caspi R , SRI International

Enzymatic reaction of: precorrin-5 (C1)-methyltransferase

EC Number:

precorrin-5 + S-adenosyl-L-methionine + H2O <=> precorrin-6A + S-adenosyl-L-homocysteine + acetate + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: adenosylcobalamin biosynthesis II (late cobalt incorporation) , cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)


Crouzet90: Crouzet J, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Thibaut D, Debussche L (1990). "Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid." J Bacteriol 172(10);5980-90. PMID: 2211521

Debussche93: Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F (1993). "Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans." J Bacteriol 1993;175(22);7430-40. PMID: 8226690

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc14.