|Gene:||puuD||Accession Numbers: G-2243 (MetaCyc), PA2366|
Species: Pseudomonas aeruginosa
The ability of Pseudomonas aeruginosa to decompose urate was not constitutive, and developed about two hours after exposure to urate, suggesting that the enzyme is inducible [Bachrach57]. 3 atoms of oxygen were taken up for every mole of urate which disappeared.
In a later experiment the activity of factor-independent urate hydroxylase from Pseudomonas aeruginosa was measured using cell extracts, by following the disappearance of urate. Specific activity was 0.065 μmol/min/mg protein [Bongaerts77].
Gene Citations: [Stover00a]
Molecular Weight of Polypeptide: 55.042 kD (from nucleotide sequence)
|Cellular Component:||GO:0016020 - membrane [Bongaerts77]|
Enzymatic reaction of: factor-independent urate hydroxylase
Synonyms: uricase, urate oxidase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
In Pathways: urate degradation to allantoin I
pH(opt): 7.5 [Bongaerts77]
Saeed04: Saeed HM, Abdel-Fattah YR, Berekaa MM, Gohar YM, Elbaz MA (2004). "Identification, cloning and expression of Pseudomonas aeruginosa Ps-x putative urate oxidase gene in Escherichia coli." Pol J Microbiol 53(4);227-36. PMID: 15790071
Stover00a: Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV (2000). "Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen." Nature 406(6799);959-64. PMID: 10984043
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