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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: factor-independent urate hydroxylase

Gene: puuD Accession Numbers: G-2243 (MetaCyc), PA2366

Synonyms: uricase

Species: Pseudomonas aeruginosa

Summary:
The ability of Pseudomonas aeruginosa to decompose urate was not constitutive, and developed about two hours after exposure to urate, suggesting that the enzyme is inducible [Bachrach57]. 3 atoms of oxygen were taken up for every mole of urate which disappeared.

In a later experiment the activity of factor-independent urate hydroxylase from Pseudomonas aeruginosa was measured using cell extracts, by following the disappearance of urate. Specific activity was 0.065 μmol/min/mg protein [Bongaerts77].

The puuD gene encoding the protein has been isolated from a genomic library. The gene was cloned and expressed in Escherichia coli and showed an uricolytic activity [Saeed04].

Gene Citations: [Stover00]

Locations: membrane

Molecular Weight of Polypeptide: 55.042 kD (from nucleotide sequence)

Unification Links: Entrez-gene:882284 , UniProt:Q6RZZ8 , UniProt:Q9I1B3

Relationship Links: InterPro:IN-FAMILY:IPR010269 , Pfam:IN-FAMILY:PF05943

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0016020 - membrane [Bongaerts77]

Credits:
Revised 22-Jan-2010 by Caspi R , SRI International


Enzymatic reaction of: factor-independent urate hydroxylase

Synonyms: uricase, urate oxidase

EC Number: 1.7.3.3

urate + oxygen + H2O <=> 5-hydroxyisourate + hydrogen peroxide

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: urate degradation to allantoin I

Inhibitors (Competitive): dinitrophenylhydrazine [Bachrach57] , semicarbazide [Bachrach57] , p-chloromercuribenzoate [Bachrach57]

Inhibitors (Unknown Mechanism): iodoacetate [Bachrach57] , benzimidazole [Bachrach57]

pH(opt): 7.5 [Bongaerts77]


References

Bachrach57: Bachrach, U (1957). "The aerobic breakdown of uric acid by certain pseudomonads." J Gen Microbiol 17(1);1-11. PMID: 13475666

Bongaerts77: Bongaerts GP, Sin IL, Peters AL, Vogels GD (1977). "Purine degradation in Pseudomonas aeruginosa and Pseudomonas testosteroni." Biochim Biophys Acta 499(1);111-8. PMID: 407941

Saeed04: Saeed HM, Abdel-Fattah YR, Berekaa MM, Gohar YM, Elbaz MA (2004). "Identification, cloning and expression of Pseudomonas aeruginosa Ps-x putative urate oxidase gene in Escherichia coli." Pol J Microbiol 53(4);227-36. PMID: 15790071

Stover00: Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV (2000). "Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen." Nature 406(6799);959-64. PMID: 10984043


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc14.