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MetaCyc Enzyme: methylbutenol synthase

Species: Pinus sabiniana

Summary:
An enzyme preparation from pine needles of Pinus sabiniana was partially purified and characterized for its biochemical properties. The enzyme catalyzes the conversion of dimethylallyl diphosphate to 2-methylbut-3-en-2-ol via the methylerythritol phosphate pathway I. This enzyme is distinct from the analogous linalool synthase, as it carries no specificity for geranyl diphosphate [Fisher00]. The enzyme requires Mn2+ for activity.

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 26-Sep-2011 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: methylbutenol synthase

dimethylallyl diphosphate + H2O <=> 2-methylbut-3-en-2-ol + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

In Pathways: methylbutenol biosynthesis

Kinetic Parameters:

Substrate
Km (μM)
Citations
dimethylallyl diphosphate
4.8
[Fisher00]

pH(opt): 7.5 [Fisher00]


References

Fisher00: Fisher AJ, Baker BM, Greenberg JP, Fall R (2000). "Enzymatic synthesis of methylbutenol from dimethylallyl diphosphate in needles of Pinus sabiniana." Arch Biochem Biophys 383(1);128-34. PMID: 11097185


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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Aug 4, 2015, biocyc13.