|Gene:||SauT||Accession Number: G-12176 (MetaCyc)|
Species: Ralstonia eutropha H16
The subunit structure of this enzyme has not been reported.
Cell extracts of Ralstonia eutropha H16 grown with sulfoacetate as the sole source of carbon and energy showed sulfoacetate-CoA ligase activity. Extracts of cells grown with acetate, taurine, or isethionate showed no such activity. This sulfoacetate-inducible enzyme was partially purified from cell extracts, where it was found in the soluble fraction. However instability prevented its further purification. Gene transcription studies confirmed the inducible nature of the enzyme. An in-frame deletion mutant was constructed which showed no activity [Weinitschke10].
Gene Citations: [Pohlmann06]
Molecular Weight of Polypeptide: 54.259 kD (from nucleotide sequence)
Relationship Links: Entrez-Nucleotide:PART-OF:AM260479 , InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR020845 , InterPro:IN-FAMILY:IPR025110 , Pfam:IN-FAMILY:PF00501 , Pfam:IN-FAMILY:PF13193 , Prosite:IN-FAMILY:PS00455
Enzymatic reaction of: sulfoacetate-CoA ligase
EC Number: 6.2.1.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
In Pathways: sulfoacetate degradation
Pohlmann06: Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Potter M, Schwartz E, Strittmatter A, Voss I, Gottschalk G, Steinbuchel A, Friedrich B, Bowien B (2006). "Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16." Nat Biotechnol 24(10);1257-62. PMID: 16964242
Weinitschke10: Weinitschke S, Hollemeyer K, Kusian B, Bowein B, Smits TH, Cook AM (2010). "Sulfoacetate is degraded via a novel pathway involving sulfoacetyl-CoA and sulfoacetaldehyde in Cupriavidus necator H16." J Biol Chem. PMID: 20693281
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493