MetaCyc Enzyme: sulfoacetate-CoA ligase (AMP-forming)

Gene: SauT Accession Number: G-12176 (MetaCyc)

Synonyms: H16_A2748

Species: Ralstonia eutropha H16

The subunit structure of this enzyme has not been reported.

Cell extracts of Ralstonia eutropha H16 grown with sulfoacetate as the sole source of carbon and energy showed sulfoacetate-CoA ligase activity. Extracts of cells grown with acetate, taurine, or isethionate showed no such activity. This sulfoacetate-inducible enzyme was partially purified from cell extracts, where it was found in the soluble fraction. However instability prevented its further purification. Gene transcription studies confirmed the inducible nature of the enzyme. An in-frame deletion mutant was constructed which showed no activity [Weinitschke10].

Gene Citations: [Pohlmann06]

Molecular Weight of Polypeptide: 54.259 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q0K844 , String:381666.H16_A2748 , UniProt:Q0K844

Relationship Links: Entrez-Nucleotide:PART-OF:AM260479 , InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR020845 , InterPro:IN-FAMILY:IPR025110 , Pfam:IN-FAMILY:PF00501 , Pfam:IN-FAMILY:PF13193 , Prosite:IN-FAMILY:PS00455

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 15-Sep-2010 by Fulcher CA , SRI International

Enzymatic reaction of: sulfoacetate-CoA ligase

EC Number: 6.2.1.-

sulfoacetate + ATP + coenzyme A <=> sulfoacetyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: sulfoacetate degradation

Enzyme activity was estimated as the decrease in coenzyme A concentration. Partially purified enzyme showed sulfoacetate-dependent formation of sulfoacetyl-CoA and AMP [Weinitschke10].

Cofactors or Prosthetic Groups: Mg2+ [Weinitschke10]


Pohlmann06: Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Potter M, Schwartz E, Strittmatter A, Voss I, Gottschalk G, Steinbuchel A, Friedrich B, Bowien B (2006). "Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16." Nat Biotechnol 24(10);1257-62. PMID: 16964242

Weinitschke10: Weinitschke S, Hollemeyer K, Kusian B, Bowein B, Smits TH, Cook AM (2010). "Sulfoacetate is degraded via a novel pathway involving sulfoacetyl-CoA and sulfoacetaldehyde in Cupriavidus necator H16." J Biol Chem 285(46);35249-54. PMID: 20693281

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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