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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
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MetaCyc Enzyme: theobromine demethylase

Species: Pseudomonas putida No. 352

Subunit composition of theobromine demethylase = [theobromine demethylase subunit]6

Summary:
The native apparent molecular mass was determined by gel filtration chromatography [Asano94].

Cloning of the gene encoding this enzyme has not been reported.

This monooxygenase was shown to be specific for theobromine demethylation at the N3 position to produce 7-methylxanthine. Based on its absorption at 415 nm and brown color in solution, it was suggested to contain either heme or nonheme iron. Pseudomonas putida No. 352 also showed high caffeine degradation activity when grown in the presence of Fe3+ [Asano94].

A soluble, NADH-dependent caffeine demethylating monooxygenase activity was also found in extracts of this organism and the formation of formaldehyde was demonstrated. In contrast to this enzyme, the caffeine demethylase activity was not inhibited by Zn2+ [Asano94].
The subunit apparent molecular mass was determined by SDS-PAGE [Asano94].

Molecular Weight of Polypeptide: 41.0 kD (experimental) [Asano94 ]

Molecular Weight of Multimer: 250.0 kD (experimental) [Asano94]

Gene-Reaction Schematic: ?

Credits:
Created 06-Jul-2010 by Fulcher CA , SRI International


Enzymatic reaction of: theobromine N3-demethylase (theobromine demethylase)

theobromine + NAD(P)H + oxygen + H+ <=> 7-methylxanthine + formaldehyde + NAD(P)+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: caffeine degradation III (bacteria, via demethylation)

Summary:
The assay for this enzyme activity utilized NADH. Whether or not the enzyme could utilize NADPH was not reported.

Inhibitors (Unknown Mechanism): Zn2+ [Asano94, Asano93]

Kinetic Parameters:

Substrate
Km (μM)
Citations
theobromine
1100.0
[Asano94]


References

Asano93: Asano Y, Komeda T, Yamada H (1993). "Microbial production of theobromine from caffeine." Biosci. Biotech. Biochem. 57 (8) , 1286-1289.

Asano94: Asano Y, Komeda T, Yamada H (1994). "Enzymes involved in theobromine production from caffeine by Pseudomonas putida No. 352." Biosci. Biotech. Biochem., 58 (12), 2303-2304.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, BIOCYC14B.