Synonyms: sulfenate dehydrogenase, lachrymatory-factor synthase
Species: Petiveria alliacea
Subunit composition of sulfenic acid dehydrogenase = [lachrymatory-factor synthase α subunit]2[lachrymatory-factor synthase γ subunit][lachrymatory-factor synthase δ subunit]
The Amazonian medicinal plant Petiveria alliacea contains a unique sulfine lachrymator, (Z)-phenylmethanethial S-oxide, which is produced by a unique lachrymatory-factor synthase. The enzyme acts on phenylmethanesulfenate, which is produced by the (Z)-phenylmethanethial S-oxide alliinase from petiveriin [Musah09].
Unlike the enzyme from onion, which was reported to contain only one type of subunit (of 19kDa), the enzyme from Petiveria alliacea is a heterotetrameric glycoprotein comprised of two α-subunits (68.8 kD each), one γ-subunit (22.5 kD), and one δ-subunit (11.9 kD). The α subunits are glycosylated and linked together by a disulfide bridge [Musah09]. In addition, while the enzyme from onion is an isomerase that catalyzes the rearrangement of (E) 1-propenylsulfenate to (Z)-propanethial S-oxide, the enzyme from Petiveria alliacea is a dehydrogenase. Enzymatic activity was enhanced in the presence of NAD+ or NADP+, but not in the presnce of FAD of FMN | [He11].
Molecular Weight: 172.0 kD (experimental) [Musah09 ]
pI: 5.2 [Musah09]
Enzymatic reaction of: phenylmethanesulfenate dehydrogenase (sulfenic acid dehydrogenase)
Synonyms: (Z)-phenylmethanethial S-oxide synthase
EC Number: 1.1.1.M1
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: (Z)-phenylmethanethial S-oxide biosynthesis
Molecular Weight: 68.8 kD (experimental) [Musah09]
Molecular Weight: 22.5 kD (experimental) [Musah09]
Molecular Weight: 11.9 kD (experimental) [Musah09]
He11: He Q, Kubec R, Jadhav AP, Musah RA (2011). "First insights into the mode of action of a "lachrymatory factor synthase" - Implications for the mechanism of lachrymator formation in Petiveria alliacea, Allium cepa and Nectaroscordum species." Phytochemistry 72(16);1939-46. PMID: 21840558
Musah09: Musah RA, He Q, Kubec R (2009). "Discovery and characterization of a novel lachrymatory factor synthase in Petiveria alliacea and its influence on alliinase-mediated formation of biologically active organosulfur compounds." Plant Physiol 151(3);1294-303. PMID: 19692535
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