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MetaCyc Enzyme: type III polyketide synthase

Gene: PKS4 Accession Number: G-11462 (MetaCyc)

Species: Aloe arborescens

Summary:
A cDNA clone was isolated from aloe plants by cDNA library screening. The cDNA was recombinantly expressed in E.coli and functionally characterized, the cDNA encodes a sequence, PKS4, that shares 85-96% identity with the previously reported octaketide synthase. This enzymes produces the shunts SEK4 and SEK4b octoketides. The enzyme catalyzes the sequential condensations of eight molecules of malonyl-CoA to produce octaketides [Mizuuchi09].

Molecular Weight of Polypeptide: 44.3 kD (from nucleotide sequence), 44.0 kD (experimental) [Mizuuchi09 ]

Unification Links: Entrez-Nucleotide:FJ536166 , Entrez:ACR19997 , UniProt:C4NF90

Relationship Links: InterPro:IN-FAMILY:IPR001099 , InterPro:IN-FAMILY:IPR011141 , InterPro:IN-FAMILY:IPR012328 , InterPro:IN-FAMILY:IPR016038 , InterPro:IN-FAMILY:IPR016039 , Pfam:IN-FAMILY:PF00195 , Pfam:IN-FAMILY:PF02797 , Prosite:IN-FAMILY:PS00441

Gene-Reaction Schematic: ?

Credits:
Created 07-Aug-2009 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: octoketide 4b synthase (type III polyketide synthase)

8 malonyl-CoA + 7 H+ <=> octoketide 4b + 8 CO2 + 8 coenzyme A + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: barbaloin biosynthesis


Enzymatic reaction of: octoketide synthase (type III polyketide synthase)

EC Number: 2.3.1.-

8 malonyl-CoA + 7 H+ <=> octoketide + 8 CO2 + 8 coenzyme A + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: barbaloin biosynthesis


References

Mizuuchi09: Mizuuchi Y, Shi SP, Wanibuchi K, Kojima A, Morita H, Noguchi H, Abe I (2009). "Novel type III polyketide synthases from Aloe arborescens." FEBS J 276(8);2391-401. PMID: 19348024


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Mar 5, 2015, biocyc13.