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MetaCyc Enzyme: taxadiene synthase

Gene: TDC1 Accession Number: G-11314 (MetaCyc)

Synonyms: taxadiene synthase

Species: Taxus wallichiana chinensis

Summary:
A cDNA clone was isolated from Taxus wallichiana chinensis.The protein was heterologously overexpressed in E.coli and shown to function as a taxadiene synthase. It catalyzes the cyclization of the isoprenoid intermediate geranylgeranyl diphosphate to taxa-4,11-diene [Wang02a]. The taxadiene synthase of Taxus wallichiana chinensis showed an overall identity with the Taxus brevifolia taxadiene synthase of 97% also containing an presumptive plastidal transit peptide [Wildung96] .

Molecular Weight of Polypeptide: 97.9 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:AY007207 , Entrez:AAG02257 , Protein Model Portal:Q9FT37 , UniProt:Q9FT37

Relationship Links: InterPro:IN-FAMILY:IPR001906 , InterPro:IN-FAMILY:IPR005630 , InterPro:IN-FAMILY:IPR008930 , InterPro:IN-FAMILY:IPR008949 , Pfam:IN-FAMILY:PF01397 , Pfam:IN-FAMILY:PF03936

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 15-May-2009 by Pujar A , Boyce Thompson Institute
Revised 07-Jun-2012 by Foerster H , Boyce Thompson Institute


Enzymatic reaction of: taxadiene synthase

EC Number: 4.2.3.17

geranylgeranyl diphosphate <=> taxa-4,11-diene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: taxol biosynthesis

Summary:
This enzyme binds 3 Mg+ ions per subunit.


References

Wang02a: Wang W, Song S, Cheng K (2002). "Cloning of a taxa-4(20),11(12)-dien-5α-ol-O-acetyltransferase cDNA from Taxus chinensis and functional expression in Escherichia coli." UniProt Q8S9G6, GenBank AAL7854.

Wildung96: Wildung MR, Croteau R (1996). "A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis." J Biol Chem 271(16);9201-4. PMID: 8621577


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Fri Mar 27, 2015, biocyc13.