Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: taxadiene synthase

Gene: TDC1 Accession Number: G-11314 (MetaCyc)

Synonyms: taxadiene synthase

Species: Taxus wallichiana chinensis

Summary:
A cDNA clone was isolated from Taxus wallichiana chinensis.The protein was heterologously overexpressed in E.coli and shown to function as a taxadiene synthase. It catalyzes the cyclization of the isoprenoid intermediate geranylgeranyl diphosphate to taxa-4,11-diene [Wang02b]. The taxadiene synthase of Taxus wallichiana chinensis showed an overall identity with the Taxus brevifolia taxadiene synthase of 97% also containing an presumptive plastidal transit peptide [Wildung96] .

Molecular Weight of Polypeptide: 97.9 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:AY007207 , Entrez:AAG02257 , Protein Model Portal:Q9FT37 , UniProt:Q9FT37

Relationship Links: InterPro:IN-FAMILY:IPR001906 , InterPro:IN-FAMILY:IPR005630 , InterPro:IN-FAMILY:IPR008930 , InterPro:IN-FAMILY:IPR008949 , Pfam:IN-FAMILY:PF01397 , Pfam:IN-FAMILY:PF03936

Gene-Reaction Schematic: ?

Credits:
Created 15-May-2009 by Pujar A , Boyce Thompson Institute
Revised 07-Jun-2012 by Foerster H , Boyce Thompson Institute


Enzymatic reaction of: taxadiene synthase

EC Number: 4.2.3.17

geranylgeranyl diphosphate <=> taxa-4,11-diene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: taxol biosynthesis

Summary:
This enzyme binds 3 Mg+ ions per subunit.


References

Wang02b: Wang W, Song S, Cheng K (2002). "Cloning of a taxa-4(20),11(12)-dien-5α-ol-O-acetyltransferase cDNA from Taxus chinensis and functional expression in Escherichia coli." UniProt Q8S9G6, GenBank AAL7854.

Wildung96: Wildung MR, Croteau R (1996). "A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis." J Biol Chem 271(16);9201-4. PMID: 8621577


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC13B.