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Metabolic Modeling Tutorial
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MetaCyc Enzyme: pseudaminic acid synthetase

Gene: pseI Accession Number: G-11090 (MetaCyc)

Synonyms: NeuB3, Cj1317

Species: Campylobacter jejuni

Summary:
The pseI of Campylobacter jejuni was discovered based on its similarity to the neuB gene, which encodes N-acetylneuraminate synthase. The gene was cloned and expressed in Escherichia coli, and the recombinant protein was purified [Chou05]. The purified enzyme catalyzed the predicted reaction in vitro, confirming its predicted activity.

The enzyme, which is monomeric, is dependent on the presence of Mn2+ or Co2+ ions [Chou05].

Molecular Weight of Polypeptide: 38.647 kD (from nucleotide sequence), 38.67 kD (experimental) [Chou05 ]

Unification Links: Entrez-gene:905609 , Protein Model Portal:Q0P8U0 , String:192222.Cj1317 , UniProt:Q0P8U0

Relationship Links: InterPro:IN-FAMILY:IPR006190 , InterPro:IN-FAMILY:IPR013132 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR013974 , InterPro:IN-FAMILY:IPR020030 , Pfam:IN-FAMILY:PF03102 , Pfam:IN-FAMILY:PF08666 , Prosite:IN-FAMILY:PS50844 , Smart:IN-FAMILY:SM00858

Gene-Reaction Schematic: ?

Credits:
Created 26-Feb-2009 by Caspi R , SRI International


Enzymatic reaction of: pseudaminic acid synthetase

Synonyms: Pse synthetase

EC Number: 2.5.1.97

phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose + H2O <=> pseudaminate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: CMP-pseudaminate biosynthesis

Activators (Allosteric): Mn2+ [Chou05] , Co2+ [Chou05]

Primary Physiological Regulators of Enzyme Activity: Mn2+

Kinetic Parameters:

Substrate
Km (μM)
Citations
phosphoenolpyruvate
6.5
[Chou05]
2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose
9.5
[Chou05]

pH(opt): 7 [Chou05]


References

Chou05: Chou WK, Dick S, Wakarchuk WW, Tanner ME (2005). "Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase." J Biol Chem 280(43);35922-8. PMID: 16120604


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc13.