|Gene:||pseI||Accession Number: G-11090 (MetaCyc)|
Synonyms: NeuB3, Cj1317
Species: Campylobacter jejuni
The pseI of Campylobacter jejuni was discovered based on its similarity to the neuB gene, which encodes N-acetylneuraminate synthase. The gene was cloned and expressed in Escherichia coli, and the recombinant protein was purified [Chou05]. The purified enzyme catalyzed the predicted reaction in vitro, confirming its predicted activity.
Molecular Weight of Polypeptide: 38.647 kD (from nucleotide sequence), 38.67 kD (experimental) [Chou05 ]
Relationship Links: InterPro:IN-FAMILY:IPR006190 , InterPro:IN-FAMILY:IPR013132 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR013974 , InterPro:IN-FAMILY:IPR020030 , Pfam:IN-FAMILY:PF03102 , Pfam:IN-FAMILY:PF08666 , Prosite:IN-FAMILY:PS50844 , Smart:IN-FAMILY:SM00858
Enzymatic reaction of: pseudaminic acid synthetase
Synonyms: Pse synthetase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: CMP-pseudaminate biosynthesis
Primary Physiological Regulators of Enzyme Activity: Mn2+
pH(opt): 7 [Chou05]
Chou05: Chou WK, Dick S, Wakarchuk WW, Tanner ME (2005). "Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase." J Biol Chem 280(43);35922-8. PMID: 16120604
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