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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: geraniol acetyltransferase

Gene: RhAAT1 Accession Number: G-10527 (MetaCyc)

Species: Rosa hybrid cultivar

Summary:
A cDNA clone was isolated from petals of 'fragrant cloud' Rosa. The cDNA was recombinantly expressed in Escherichia coli and the molecular weight of the protein was calculated to be 51.762 kDa [Shalit03]. The cDNA encoded a protein with high similarity to BAHD family of acetyltransferase. The recombinant protein was partially purified and shown to accept alcohols like geraniol and citronellol with acetyl Coenzyme A producing the respective esters geranyl acetate and citronellyl acetate, hence acting as geraniol acetyltransferase,. The AAT1 gene was expressed only in floral tissues during flower development.

Molecular Weight of Polypeptide: 51.762 kD (from nucleotide sequence), 53.0 kD (experimental) [Shalit03 ]

pI: 5.45 [Shalit03]

Unification Links: Entrez:AAW31948 , Protein Model Portal:Q5I6B5 , UniProt:Q5I6B5

Relationship Links: Entrez-Nucleotide:PART-OF:AY850287 , InterPro:IN-FAMILY:IPR003480 , InterPro:IN-FAMILY:IPR023213 , Pfam:IN-FAMILY:PF02458

Gene-Reaction Schematic: ?

GO Terms:

Molecular Function: GO:0016407 - acetyltransferase activity Inferred from experiment [Shalit03]

Credits:
Created 06-Mar-2008 by Pujar A , Cornell University
Revised 23-Jul-2012 by Foerster H , Boyce Thompson Institute


Enzymatic reaction of: geraniol acetyltransferase

geraniol + acetyl-CoA <=> geranyl acetate + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: geranyl acetate biosynthesis

Kinetic Parameters:

Substrate
Km (μM)
Specific Activity (U/mg)
Citations
geraniol
160.0
[Shalit03]
acetyl-CoA
65.0
[Shalit03]

pH(opt): 7-8 [Shalit03]


References

Shalit03: Shalit M, Guterman I, Volpin H, Bar E, Tamari T, Menda N, Adam Z, Zamir D, Vainstein A, Weiss D, Pichersky E, Lewinsohn E (2003). "Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/Citronellol acetyltransferase in developing rose petals." Plant Physiol 131(4);1868-76. PMID: 12692346


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, biocyc14.