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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: phloroglucinol O-methyltransferase

Gene: POMT Accession Number: G-10428 (MetaCyc)

Species: Rosa chinensis

Subunit composition of phloroglucinol O-methyltransferase = [POMT]2
         phloroglucinol O-methyltransferase = POMT

Summary:
A cDNA clone was isolated from rose (Rosa chinensis) petals. The cDNA was recombinantly expressed in E.coli and the molecular weight of the protein was calculated to be 41.032 kDa. The functional analysis of the POMT protein purified on a nickel His-binding affinity column showed that it catalyzed the methylation of phloroglucinol [Wu04a]. The native protein was purified by SDS chromotography and the molecular mass determined by SDS-PAGE was 43.0kDa. However, the molecular mass of the native protein was determined at 86 kDa indicating POMT as a dimeric enzyme in its native form The partially purified enzyme exhibited the same substrate specificity as the recombinant POMT and was predominantly expressed in floral organs.

Molecular Weight of Polypeptide: 41.032 kD (from nucleotide sequence), 43.0 kD (experimental) [Wu04a ]

Molecular Weight of Multimer: 86.0 kD (experimental) [Wu04a]

Unification Links: Entrez:BAD18975 , Protein Model Portal:Q6L8K4 , SMR:Q6L8K4 , UniProt:Q6L8K4

Relationship Links: Entrez-Nucleotide:PART-OF:AB121046 , InterPro:IN-FAMILY:IPR001077 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR012967 , InterPro:IN-FAMILY:IPR016461 , Pfam:IN-FAMILY:PF00891 , Pfam:IN-FAMILY:PF08100 , Prosite:IN-FAMILY:PS51588

Gene-Reaction Schematic: ?

GO Terms:

Molecular Function: GO:0008171 - O-methyltransferase activity Inferred from experiment [Wu04a]

Credits:
Created 09-Jan-2008 by Pujar A , Cornell University
Revised 12-Jul-2012 by Foerster H , Boyce Thompson Institute


Enzymatic reaction of: phloroglucinol O-methyltransferase

EC Number: 2.1.1.-

phloroglucinol + S-adenosyl-L-methionine <=> 3,5-dihydroxyanisole + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: 1,3,5-trimethoxybenzene biosynthesis

Kinetic Parameters:

Substrate
Km (μM)
Citations
S-adenosyl-L-methionine
16.2
[Wu04a]
phloroglucinol
94.5
[Wu04a]


References

Wu04a: Wu S, Watanabe N, Mita S, Dohra H, Ueda Y, Shibuya M, Ebizuka Y (2004). "The key role of phloroglucinol O-methyltransferase in the biosynthesis of Rosa chinensis volatile 1,3,5-trimethoxybenzene." Plant Physiol 135(1);95-102. PMID: 15122041


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14A.