|Gene:||POMT||Accession Number: G-10428 (MetaCyc)|
Species: Rosa chinensis
A cDNA clone was isolated from rose (Rosa chinensis) petals. The cDNA was recombinantly expressed in E.coli and the molecular weight of the protein was calculated to be 41.032 kDa. The functional analysis of the POMT protein purified on a nickel His-binding affinity column showed that it catalyzed the methylation of phloroglucinol [Wu04a]. The native protein was purified by SDS chromotography and the molecular mass determined by SDS-PAGE was 43.0kDa. However, the molecular mass of the native protein was determined at 86 kDa indicating POMT as a dimeric enzyme in its native form The partially purified enzyme exhibited the same substrate specificity as the recombinant POMT and was predominantly expressed in floral organs.
Molecular Weight of Polypeptide: 41.032 kD (from nucleotide sequence), 43.0 kD (experimental) [Wu04a ]
Molecular Weight of Multimer: 86.0 kD (experimental) [Wu04a]
Relationship Links: Entrez-Nucleotide:PART-OF:AB121046 , InterPro:IN-FAMILY:IPR001077 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR012967 , InterPro:IN-FAMILY:IPR016461 , InterPro:IN-FAMILY:IPR029063 , Pfam:IN-FAMILY:PF00891 , Pfam:IN-FAMILY:PF08100 , Prosite:IN-FAMILY:PS51588
|Molecular Function:||GO:0008171 - O-methyltransferase activity [Wu04a]|
Enzymatic reaction of: phloroglucinol O-methyltransferase
EC Number: 2.1.1.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
In Pathways: 1,3,5-trimethoxybenzene biosynthesis
Wu04a: Wu S, Watanabe N, Mita S, Dohra H, Ueda Y, Shibuya M, Ebizuka Y (2004). "The key role of phloroglucinol O-methyltransferase in the biosynthesis of Rosa chinensis volatile 1,3,5-trimethoxybenzene." Plant Physiol 135(1);95-102. PMID: 15122041
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