MetaCyc Enzyme: (E,E)-α-farnesene synthase

Gene: AFS1 Accession Number: G-10317 (MetaCyc)

Species: Malus domestica

A cDNA clone was isolated from apple Malus domestica fruit peel tissue. The cDNA was recombinantly expressed in E.coli and the molecular weight of the protein was determined at 66 kDa [Pechous04]. The protein was shown to catalyze the conversion of (2E,6E)-farnesyl diphosphate almost exclusively into (E,E)-α-farnesene [Pechous04].

Molecular Weight of Polypeptide: 66.183 kD (from nucleotide sequence), 66.0 kD (experimental) [Pechous04 ]

Unification Links: Entrez-Nucleotide:AY182241 , Entrez:AA022848 , Entrez:AAO22848 , Protein Model Portal:Q84LB2 , UniProt:Q84LB2

Relationship Links: Entrez-Nucleotide:PART-OF:AY182241 , InterPro:IN-FAMILY:IPR001906 , InterPro:IN-FAMILY:IPR005630 , InterPro:IN-FAMILY:IPR008930 , InterPro:IN-FAMILY:IPR008949 , Pfam:IN-FAMILY:PF01397 , Pfam:IN-FAMILY:PF03936

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0016106 - sesquiterpenoid biosynthetic process Inferred from experiment [Pechous04]

Created 27-Nov-2007 by Pujar A , Cornell University

Enzymatic reaction of: (E,E)-α-farnesene synthase

EC Number:

(2E,6E)-farnesyl diphosphate <=> (E,E)-α-farnesene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: farnesene biosynthesis

pH(opt): 7 [Pechous04]


Pechous04: Pechous SW, Whitaker BD (2004). "Cloning and functional expression of an ( E, E)-alpha-farnesene synthase cDNA from peel tissue of apple fruit." Planta 219(1);84-94. PMID: 14740213

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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