Species: Azotobacter vinelandii
Molecular Weight of Polypeptide: 74 kD (experimental) [Wong94a]
pI: 6.15 [Wong94a]
Enzymatic reaction of: galactose dehydrogenase
Synonyms: galactose 1-dehydrogenase, GalDH
EC Number: 220.127.116.11D-galactopyranose + NAD+ → D-galactono-1,4-lactone + NADH + H+
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.Alternative Substrates for D-galactopyranose: L-arabinose [Wong94a]
In Pathways: D-galactose degradation II
Galactose dehydrogenase was induced by growth of cells on galactose. The reaction was highly specific for NAD+. The partially purified enzyme preparation also oxidized L-arabinose and D-fucose. L-Arabinose was a better substrate than D-galactose, and it was suggested that the L-arabinose dehydrogenase activity was an integral part of galactose dehydrogenase. The following substrates were not oxidized: D-glucose, D-fructose, D-mannose, D-ribose, and 1-deoxy-D-galactose [Wong94a].
pH(opt): 9.0 [Wong94a]
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