MetaCyc Enzyme: galactose dehydrogenase

Species: Azotobacter vinelandii

The relative molecular mass was estimated by HPLC gel filtration chromatography [Wong94a]. The subunit structure of this enzyme from Azotobacter vinelandii has not been reported.

Locations: cytosol

Molecular Weight of Polypeptide: 74 kD (experimental) [Wong94a]

pI: 6.15 [Wong94a]

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Cellular Component:
GO:0005829 - cytosol [Wong94a]

Created 12-Jan-2007 by Fulcher CA, SRI International

Enzymatic reaction of: galactose dehydrogenase

Inferred from experiment

Synonyms: galactose 1-dehydrogenase, GalDH

EC Number:

D-galactopyranose + NAD+ → D-galactono-1,4-lactone + NADH + H+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for D-galactopyranose: L-arabinose [Wong94a]

In Pathways: D-galactose degradation II

Galactose dehydrogenase was induced by growth of cells on galactose. The reaction was highly specific for NAD+. The partially purified enzyme preparation also oxidized L-arabinose and D-fucose. L-Arabinose was a better substrate than D-galactose, and it was suggested that the L-arabinose dehydrogenase activity was an integral part of galactose dehydrogenase. The following substrates were not oxidized: D-glucose, D-fructose, D-mannose, D-ribose, and 1-deoxy-D-galactose [Wong94a].

Activators (Allosteric): D-fucose [Wong94a]Kinetic Parameters:
Substrate Km (μM) Citations
D-galactopyranose 560.0 [Wong94a]
NAD+ 125.0 [Wong94a]

pH(opt): 9.0 [Wong94a]


Wong94a: Wong TY, Yao XT (1994). "The DeLey-Doudoroff Pathway of Galactose Metabolism in Azotobacter vinelandii." Appl Environ Microbiol 60(6);2065-2068. PMID: 16349292

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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