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MetaCyc Enzyme: galactose dehydrogenase

Species: Azotobacter vinelandii

Summary:
The relative molecular mass was estimated by HPLC gel filtration chromatography [Wong94]. The subunit structure of this enzyme from Azotobacter vinelandii has not been reported.

Locations: cytosol

Molecular Weight of Polypeptide: 74 kD (experimental) [Wong94 ]

pI: 6.15 [Wong94]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005829 - cytosol [Wong94]

Credits:
Created 12-Jan-2007 by Fulcher CA , SRI International


Enzymatic reaction of: galactose dehydrogenase

Synonyms: galactose 1-dehydrogenase, GalDH

EC Number: 1.1.1.48

β-D-galactose + NAD+ <=> D-galactono-1,4-lactone + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for β-D-galactose: L-arabinose [Wong94 ]

In Pathways: galactose degradation II

Summary:
Galactose dehydrogenase was induced by growth of cells on galactose. The reaction was highly specific for NAD+. The partially purified enzyme preparation also oxidized L-arabinose and D-fucose. L-Arabinose was a better substrate than D-galactose, and it was suggested that the L-arabinose dehydrogenase activity was an integral part of galactose dehydrogenase. The following substrates were not oxidized: D-glucose, D-fructose, D-mannose, D-ribose, and 1-deoxy-D-galactose [Wong94].

Activators (Allosteric): D-fucose [Wong94]

Kinetic Parameters:

Substrate
Km (μM)
Citations
β-D-galactose
560.0
[Wong94]
NAD+
125.0
[Wong94]

pH(opt): 9.0 [Wong94]


References

Wong94: Wong TY, Yao XT (1994). "The DeLey-Doudoroff Pathway of Galactose Metabolism in Azotobacter vinelandii." Appl Environ Microbiol 60(6);2065-2068. PMID: 16349292


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue May 26, 2015, BIOCYC14A.