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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: galactose dehydrogenase

Species: Azotobacter vinelandii

Summary:
The relative molecular mass was estimated by HPLC gel filtration chromatography [Wong94a]. The subunit structure of this enzyme from Azotobacter vinelandii has not been reported.

Locations: cytosol

Molecular Weight of Polypeptide: 74 kD (experimental) [Wong94a ]

pI: 6.15 [Wong94a]

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005829 - cytosol [Wong94a]

Credits:
Created 12-Jan-2007 by Fulcher CA , SRI International


Enzymatic reaction of: galactose dehydrogenase

Synonyms: galactose 1-dehydrogenase, GalDH

EC Number: 1.1.1.48

β-D-galactose + NAD+ <=> D-galactono-1,4-lactone + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for β-D-galactose: L-arabinose [Wong94a ]

In Pathways: galactose degradation II

Summary:
Galactose dehydrogenase was induced by growth of cells on galactose. The reaction was highly specific for NAD+. The partially purified enzyme preparation also oxidized L-arabinose and D-fucose. L-Arabinose was a better substrate than D-galactose, and it was suggested that the L-arabinose dehydrogenase activity was an integral part of galactose dehydrogenase. The following substrates were not oxidized: D-glucose, D-fructose, D-mannose, D-ribose, and 1-deoxy-D-galactose [Wong94a].

Activators (Allosteric): D-fucose [Wong94a]

Kinetic Parameters:

Substrate
Km (μM)
Citations
β-D-galactose
560.0
[Wong94a]
NAD+
125.0
[Wong94a]

pH(opt): 9.0 [Wong94a]


References

Wong94a: Wong TY, Yao XT (1994). "The DeLey-Doudoroff Pathway of Galactose Metabolism in Azotobacter vinelandii." Appl Environ Microbiol 60(6);2065-2068. PMID: 16349292


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, biocyc13.