MetaCyc Enzyme: galactose dehydrogenase

Species: Azotobacter vinelandii

The relative molecular mass was estimated by HPLC gel filtration chromatography [Wong94a]. The subunit structure of this enzyme from Azotobacter vinelandii has not been reported.

Locations: cytosol

Molecular Weight of Polypeptide: 74 kD (experimental) [Wong94a ]

pI: 6.15 [Wong94a]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005829 - cytosol [Wong94a]

Created 12-Jan-2007 by Fulcher CA , SRI International

Enzymatic reaction of: galactose dehydrogenase

Synonyms: galactose 1-dehydrogenase, GalDH

EC Number:

β-D-galactose + NAD+ <=> D-galactono-1,4-lactone + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for β-D-galactose: L-arabinose [Wong94a ]

In Pathways: galactose degradation II

Galactose dehydrogenase was induced by growth of cells on galactose. The reaction was highly specific for NAD+. The partially purified enzyme preparation also oxidized L-arabinose and D-fucose. L-Arabinose was a better substrate than D-galactose, and it was suggested that the L-arabinose dehydrogenase activity was an integral part of galactose dehydrogenase. The following substrates were not oxidized: D-glucose, D-fructose, D-mannose, D-ribose, and 1-deoxy-D-galactose [Wong94a].

Activators (Allosteric): D-fucose [Wong94a]

Kinetic Parameters:

Km (μM)

pH(opt): 9.0 [Wong94a]


Wong94a: Wong TY, Yao XT (1994). "The DeLey-Doudoroff Pathway of Galactose Metabolism in Azotobacter vinelandii." Appl Environ Microbiol 60(6);2065-2068. PMID: 16349292

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, biocyc13.