|Gene:||trmH||Accession Numbers: EG10967 (MetaCyc), b3651, ECK3641|
Species: Escherichia coli K-12 substr. MG1655
TrmH is the methyltransferase responsible for 2'-O-methylation of ribose at position G18 in certain tRNAs [Persson97a]. Unlike Type I enzymes (e.g. from Thermus thermophilus) that can modify all tRNA species, the E. coli enzyme is a Type II enzyme that only modifies a subset of tRNAs. The catalytic domain of TrmH is responsible for substrate selectivity [Ochi13].
[Persson97a] reported no growth defect of a trmH in LB or MOPS-glucose medium, while [Urbonavicius02] found a lower growth rate in rich-MOPS medium compared to wild type. The ΔspoU3 insertion-deletion allele of trmH causes phenotypes due to polar effects on recG gene expression [Kalman92]. A trmH trmA truB triple mutant shows a defect in the tRNA modifications Gm18, m5U54, and Ψ55 as well as translation defects, heat sensitivity, and a growth defect [Urbonavicius02].
TrmH: "tRNA methylation" [Persson97a]
|Map Position: [3,822,538 -> 3,823,227]|
Molecular Weight of Polypeptide: 25.343 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0011937 , CGSC:33877 , DIP:DIP-35977N , EchoBASE:EB0960 , EcoGene:EG10967 , EcoliWiki:b3651 , ModBase:P0AGJ2 , OU-Microarray:b3651 , PortEco:trmH , PR:PRO_000024115 , Pride:P0AGJ2 , Protein Model Portal:P0AGJ2 , RefSeq:NP_418108 , RegulonDB:EG10967 , SMR:P0AGJ2 , String:511145.b3651 , Swiss-Model:P0AGJ2 , UniProt:P0AGJ2
|Biological Process:||GO:0002938 - tRNA guanine ribose methylation
GO:0006396 - RNA processing [GOA01a]
GO:0008033 - tRNA processing [UniProtGOA11a]
GO:0030488 - tRNA methylation [Persson97a]
GO:0032259 - methylation [UniProtGOA11a]
|Molecular Function:||GO:0009020 - tRNA (guanosine-2'-O-)-methyltransferase activity
[GOA01, GOA01a, Ochi13, Persson97a]
GO:0003723 - RNA binding [GOA01a]
GO:0008168 - methyltransferase activity [UniProtGOA11a]
GO:0008173 - RNA methyltransferase activity [GOA01a]
GO:0016740 - transferase activity [UniProtGOA11a]
|Cellular Component:||GO:0005737 - cytoplasm
GO:0005829 - cytosol [DiazMejia09]
|MultiFun Terms:||information transfer → RNA related → RNA modification|
Enzymatic reaction of: tRNA (Gm18) 2'-O-methyltransferase
Synonyms: tRNA guanosine 2'-methyltransferase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
10/20/97 Gene b3651 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10967; confirmed by SwissProt match.
Anantharaman02a: Anantharaman V, Koonin EV, Aravind L (2002). "SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases." J Mol Microbiol Biotechnol 4(1);71-5. PMID: 11763972
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Gehrig12: Gehrig S, Eberle ME, Botschen F, Rimbach K, Eberle F, Eigenbrod T, Kaiser S, Holmes WM, Erdmann VA, Sprinzl M, Bec G, Keith G, Dalpke AH, Helm M (2012). "Identification of modifications in microbial, native tRNA that suppress immunostimulatory activity." J Exp Med 209(2);225-33. PMID: 22312113
Gustafsson96: Gustafsson C, Reid R, Greene PJ, Santi DV (1996). "Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes." Nucleic Acids Res 24(19);3756-62. PMID: 8871555
Jockel12: Jockel S, Nees G, Sommer R, Zhao Y, Cherkasov D, Hori H, Ehm G, Schnare M, Nain M, Kaufmann A, Bauer S (2012). "The 2'-O-methylation status of a single guanosine controls transfer RNA-mediated Toll-like receptor 7 activation or inhibition." J Exp Med 209(2);235-41. PMID: 22312111
Kinghorn02: Kinghorn SM, O'Byrne CP, Booth IR, Stansfield I (2002). "Physiological analysis of the role of truB in Escherichia coli: a role for tRNA modification in extreme temperature resistance." Microbiology 148(Pt 11);3511-20. PMID: 12427942
Ochi13: Ochi A, Makabe K, Yamagami R, Hirata A, Sakaguchi R, Hou YM, Watanabe K, Nureki O, Kuwajima K, Hori H (2013). "The Catalytic Domain of Topological Knot tRNA Methyltransferase (TrmH) Discriminates between Substrate tRNA and Nonsubstrate tRNA via an Induced-fit Process." J Biol Chem 288(35);25562-74. PMID: 23867454
Persson97a: Persson BC, Jager G, Gustafsson C (1997). "The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity." Nucleic Acids Res 25(20);4093-7. PMID: 9321663
Urbonavicius02: Urbonavicius J, Durand JM, Bjork GR (2002). "Three modifications in the D and T arms of tRNA influence translation in Escherichia coli and expression of virulence genes in Shigella flexneri." J Bacteriol 184(19);5348-57. PMID: 12218021
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