MetaCyc Enzyme: 3-sulfolactate sulfo-lyase

Species: Paracoccus pantotrophus NKNCYSA

Subunit composition of 3-sulfolactate sulfo-lyase = [SuyA][SuyB]
         3-sulfolactate sulfo-lyase small subunit = SuyA (summary available)
         3-sulfolactate sulfo-lyase large subunit = SuyB (summary available)

The subunit coefficients in this enzyme complex from Paracoccus pantotrophus NKNCYSA have not been reported.

The nitrite-reducing strain Paracoccus pantotrophus NKNCYSA is able to utilize 3-sulfolactate (racemic sulfolactate) and L-cysteate, presumably via a membrane transport system. A pathway for L-cysteate degradation has been proposed in this organism that involves sulfolactate as an intermediate and a substrate for this desulfonating enzyme.

Enzyme activity was present in cells grown with L-cysteate or 3-sulfolactate, but not in cells grown with acetate, taurine, isethionate, or sulfoacetate, indicating an inducible enzyme [Rein05]. The 3-sulfolactate sulfo-lyase enzyme is a heterodimer, composed of a 42 kDa and an 8 kDa subunits. Both subunits were purified to homogeneity, and the purified protein catalyzed the sulfolactate sulfo-lyase reaction [Rein05].

The enantiomeric preference of the enzyme from Paracoccus pantotrophus NKNCYSA has not been established, although an enantiomeric specificity was inferred, along with an inferred racemization step in the pathway [Rein05]. Desulfonation is a key step in bacterial utilization of its carbon and sulfur moieties (as described in the summary for pathway sulfolactate degradation I).

Hypothetical, contiguous suyAB-like genes were found in several different genomes of bacteria, archaea and environmental samples grown with 3-sulfolactate [Rein05].

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 23-Sep-2010 by Fulcher CA, SRI International

Enzymatic reaction of: 3-sulfolactate sulfo-lyase

Inferred from experiment

EC Number:

(2R)-3-sulfolactate → pyruvate + sulfite + H+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: (R)-cysteate degradation

The assay used for this enzyme was based the disappearance of 3-sulfolactate (racemic sulfolactate) with stoichiometric formation of pyruvate and hydrogen sulfite (sulfite). Experiments using crude extracts suggested a divalent cation requirement of Fe2+, Mn2+ or Co2+ [Rein05].

Subunit of 3-sulfolactate sulfo-lyase: 3-sulfolactate sulfo-lyase small subunit

Synonyms: SuyA

Gene: suyA Accession Number: G-12195 (MetaCyc)

Molecular Weight: 7.328 kD (from nucleotide sequence)

Molecular Weight: 8.0 kD (experimental) [Rein05]

Unification Links: UniProt:Q58Y44

The subunit apparent molecular mass was determined by SDS-PAGE [Rein05].

Subunit of 3-sulfolactate sulfo-lyase: 3-sulfolactate sulfo-lyase large subunit

Synonyms: SuyB

Gene: suyB Accession Number: G-12194 (MetaCyc)

Molecular Weight: 42.452 kD (from nucleotide sequence)

Molecular Weight: 42.0 kD (experimental) [Rein05]

Unification Links: UniProt:Q58Y43

Relationship Links: InterPro:IN-FAMILY:IPR007392, Pfam:IN-FAMILY:PF04295

The subunit apparent molecular mass was determined by SDS-PAGE [Rein05].


Rein05: Rein U, Gueta R, Denger K, Ruff J, Hollemeyer K, Cook AM (2005). "Dissimilation of cysteate via 3-sulfolactate sulfo-lyase and a sulfate exporter in Paracoccus pantotrophus NKNCYSA." Microbiology 151(Pt 3);737-47. PMID: 15758220

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Fri Nov 27, 2015, BIOCYC13A.