|Gene:||amnE||Accession Number: G-11252 (MetaCyc)|
Species: Pseudomonas sp. AP3
Subunit composition of
4-oxalocrotonate decarboxylase = [AmnE]
4-oxalocrotonate decarboxylase monomer = AmnE
4-oxalocrotonate decarboxylase has been purified from Pseudomonas sp. AP3 to homogeneity [Takenaka00]. The protein was found to form a large complex (300 kDa) with the hydratase encoded by amnF, which catalyzes the next step of the pathway. The ratio of decarboxylase to hydratase subunits within the complex was 1:1.
Molecular Weight of Polypeptide: 27.456 kD (from nucleotide sequence), 29.0 kD (experimental) [Takenaka00 ]
Enzymatic reaction of: 4-oxalocrotonate decarboxylase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: 2-aminophenol degradation
pH(opt): 7.5 [Takenaka00]
Takenaka00: Takenaka S, Murakami S, Kim YJ, Aoki K (2000). "Complete nucleotide sequence and functional analysis of the genes for 2-aminophenol metabolism from Pseudomonas sp. AP-3." Arch Microbiol 174(4);265-72. PMID: 11081795
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