Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: malonyl CoA reductase

Gene: mcr Accession Numbers: G-15069 (MetaCyc), Caur_2614

Species: Chloroflexus aurantiacus

Subunit composition of malonyl CoA reductase = [Mcr]2
         malonyl CoA reductase subunit = Mcr

Summary:
Malonyl CoA reductase, an enzyme that catalyzes the two-step reduction of malonyl-CoA and NADPH to 3-hydroxypropanoate via malonate semialdehyde, has been isolated from Chloroflexus aurantiacus [Hugler02].

This bifunctional enzyme is a homodimer of 300 kDa, and consists of an N-terminal short-chain alcohol dehydrogenase domain and a C-terminal aldehyde dehydrogenase domain. It is highly specific for its substrates.

The N-terminal amino acid sequence was determined, and an incomplete gene sequence was identified in the genome database [Hugler02].

Gene Citations: [Tang11a]

Molecular Weight of Polypeptide: 131.98 kD (from nucleotide sequence), 145.0 kD (experimental) [Hugler02 ]

Molecular Weight of Multimer: 300.0 kD (experimental) [Hugler02]

Unification Links: Entrez-gene:5827083 , String:324602.Caur_2614 , UniProt:A9WIU3

Relationship Links: InterPro:IN-FAMILY:IPR002198 , InterPro:IN-FAMILY:IPR002347 , InterPro:IN-FAMILY:IPR016040 , Pfam:IN-FAMILY:PF00106 , Prints:IN-FAMILY:PR00080 , Prints:IN-FAMILY:PR00081

Gene-Reaction Schematic: ?

Credits:
Created 07-Dec-2007 by Caspi R , SRI International


Enzymatic reaction of: 3-hydroxypropionate:NADP+ oxidoreductase (malonyl CoA reductase)

EC Number: 1.1.1.298

3-hydroxypropanoate + NADP+ <=> malonate semialdehyde + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: superpathway of the 3-hydroxypropionate cycle , 3-hydroxypropanoate cycle , glyoxylate assimilation

Summary:
The enzyme has a turnover number of 25 s-1 per subunit. The specific activity in autotrophically grown cells was 0.08 μmol of malonyl-CoA reduced per min per mg protein. The value is much lower in heterotrophically grown cells, indicating downregulation under heterotrophic conditions. Specific activity of the purified protein was 10 μmol per min per mg protein [Hugler02].


Enzymatic reaction of: malonate semialdehyde:NADP+ oxidoreductase (malonate semialdehyde-forming) (malonyl CoA reductase)

EC Number: 1.2.1.75

malonate semialdehyde + coenzyme A + NADP+ <=> malonyl-CoA + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

In Pathways: superpathway of the 3-hydroxypropionate cycle , 3-hydroxypropanoate cycle , glyoxylate assimilation

Activators (Allosteric): Mg2+ [Hugler02] , Fe2+ [Hugler02] , Ca2+ [Hugler02]

Kinetic Parameters:

Substrate
Km (μM)
Citations
malonyl-CoA
30.0
[Hugler02]
NADPH
25.0
[Hugler02]

pH(opt): 7.8 [Hugler02]


References

Hugler02: Hugler M, Menendez C, Schagger H, Fuchs G (2002). "Malonyl-coenzyme A reductase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO(2) fixation." J Bacteriol 184(9);2404-10. PMID: 11948153

Tang11a: Tang KH, Barry K, Chertkov O, Dalin E, Han CS, Hauser LJ, Honchak BM, Karbach LE, Land ML, Lapidus A, Larimer FW, Mikhailova N, Pitluck S, Pierson BK, Blankenship RE (2011). "Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus." BMC Genomics 12;334. PMID: 21714912


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC13A.