|Gene:||nbaA||Accession Number: G-10159 (MetaCyc)|
Species: Pseudomonas fluorescens KU-7
Subunit composition of
2-nitrobenzoate nitroreductase = [NbaA]2
2-nitrobenzoate nitroreductase subunit = NbaA
The specific activity of the recombinant enzyme, which was shown to be a homodimer, was measured in cell-free extracts of the heterologous host, and found to be 10 U/mg of protein.
The enzyme activity was augmented by the presence of a flavin (especially FMN) and a divalent metal ion (best results were obtained by adding 0.3 mM of Mn2+ and Fe2+, which increased activity by 89% and 66%, respectively).
Mutational analysis supported a role of Asn40, Asp76, and Glu113, which are predicted to form the binding site for a divalent metal ion implicated in FMN binding, and a role in NADPH binding for the 10-residue insertion in the β5-α2 loop [Iwaki07].
Molecular Weight of Polypeptide: 24.411 kD (from nucleotide sequence), 28.0 kD (experimental) [Iwaki07 ]
Molecular Weight of Multimer: 45.0 kD (experimental) [Iwaki07]
Enzymatic reaction of: 2-nitrobenzoate nitroreductase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is favored in the direction shown.
In Pathways: 2-nitrobenzoate degradation I
Iwaki07: Iwaki H, Muraki T, Ishihara S, Hasegawa Y, Rankin KN, Sulea T, Boyd J, Lau PC (2007). "Characterization of a pseudomonad 2-nitrobenzoate nitroreductase and its catabolic pathway-associated 2-hydroxylaminobenzoate mutase and a chemoreceptor involved in 2-nitrobenzoate chemotaxis." J Bacteriol 189(9);3502-14. PMID: 17277060
Muraki03: Muraki T, Taki M, Hasegawa Y, Iwaki H, Lau PC (2003). "Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7." Appl Environ Microbiol 69(3);1564-72. PMID: 12620844
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