Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: glyceraldehyde 3-phosphate dehydrogenase

Species: Haloarcula vallismortis

Subunit composition of glyceraldehyde 3-phosphate dehydrogenase = [glyceraldehyde 3-phosphate dehydrogenase subunit]4

Summary:
The native molecular mass was estimated by gel filtration chromatography in the presence of 2 molar salt [Pruss93]. The enzyme from this species was determined to be more similar in amino acid sequence to eukaryotes and eubacteria than to enzymes of other archaebacteria [Pruss93].
The subunit relative molecular mass was determined by CTAB-PAGE. This technique is preferable to SDS-PAGE for halophilic proteins [Pruss93].

Molecular Weight of Polypeptide: 38 kD (experimental) [Pruss93 ]

Molecular Weight of Multimer: 160 kD (experimental) [Pruss93]

pI: 4.25 [Pruss93]

Gene-Reaction Schematic: ?

Credits:
Created 10-Nov-2006 by Fulcher CA , SRI International


Enzymatic reaction of: glyceraldehyde 3-phosphate dehydrogenase

EC Number: 1.2.1.12

D-glyceraldehyde 3-phosphate + NAD+ + phosphate <=> 1,3-bisphospho-D-glycerate + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

Summary:
The enzyme was shown to be strictly NAD+-dependent and its activity was highest in the presence of 2-3 molar potassium chloride. Arsenate was an alternate substrate for phosphate [Pruss93].

Inhibitors (Unknown Mechanism): iodoacetamide [Krishnan90] , p-chloromercuribenzoate [Krishnan90] , Hg2+ [Krishnan90] , Cu2+ [Krishnan90]

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-glyceraldehyde 3-phosphate
150.0
[Pruss93]
phosphate
1250.0
[Pruss93]
NAD+
510.0
[Pruss93]

pH(opt): 8.6 [Krishnan90]


References

Krishnan90: Krishnan G, Altekar W (1990). "Characterization of a halophilic glyceraldehyde-3-phosphate dehydrogenase from the archaebacterium Haloarcula vallismortis." J. Gen. Appl. Microbiol. 36:19-32.

Pruss93: Pruss B, Meyer HE, Holldorf AW (1993). "Characterization of the glyceraldehyde 3-phosphate dehydrogenase from the extremely halophilic archaebacterium Haloarcula vallismortis." Arch Microbiol 160(1);5-11. PMID: 8352651


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14A.