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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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MetaCyc Enzyme: glyceraldehyde 3-phosphate dehydrogenase

Species: Haloarcula vallismortis

Subunit composition of glyceraldehyde 3-phosphate dehydrogenase = [glyceraldehyde 3-phosphate dehydrogenase subunit]4

Summary:
The native molecular mass was estimated by gel filtration chromatography in the presence of 2 molar salt [Pruss93]. The enzyme from this species was determined to be more similar in amino acid sequence to eukaryotes and eubacteria than to enzymes of other archaebacteria [Pruss93].
The subunit relative molecular mass was determined by CTAB-PAGE. This technique is preferable to SDS-PAGE for halophilic proteins [Pruss93].

Molecular Weight of Polypeptide: 38 kD (experimental) [Pruss93 ]

Molecular Weight of Multimer: 160 kD (experimental) [Pruss93]

pI: 4.25 [Pruss93]

Gene-Reaction Schematic: ?

Credits:
Created 10-Nov-2006 by Fulcher CA , SRI International


Enzymatic reaction of: glyceraldehyde 3-phosphate dehydrogenase

EC Number: 1.2.1.12

D-glyceraldehyde 3-phosphate + NAD+ + phosphate <=> 1,3-bisphospho-D-glycerate + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

Summary:
The enzyme was shown to be strictly NAD+-dependent and its activity was highest in the presence of 2-3 molar potassium chloride. Arsenate was an alternate substrate for phosphate [Pruss93].

Inhibitors (Unknown Mechanism): iodoacetamide [Krishnan90] , p-chloromercuribenzoate [Krishnan90] , Hg2+ [Krishnan90] , Cu2+ [Krishnan90]

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-glyceraldehyde 3-phosphate
150.0
[Pruss93]
phosphate
1250.0
[Pruss93]
NAD+
510.0
[Pruss93]

pH(opt): 8.6 [Krishnan90]


References

Krishnan90: Krishnan G, Altekar W (1990). "Characterization of a halophilic glyceraldehyde-3-phosphate dehydrogenase from the archaebacterium Haloarcula vallismortis." J. Gen. Appl. Microbiol. 36:19-32.

Pruss93: Pruss B, Meyer HE, Holldorf AW (1993). "Characterization of the glyceraldehyde 3-phosphate dehydrogenase from the extremely halophilic archaebacterium Haloarcula vallismortis." Arch Microbiol 160(1);5-11. PMID: 8352651


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc12.