|Gene:||sucP||Accession Number: G-9706 (MetaCyc)|
Species: Bifidobacterium adolescentis
Subunit composition of
sucrose phosphorylase = [SucP]2
sucrose phosphorylase subunit = SucP
The recombinant enzyme was expressed in Escherichia coli and purified. The native molecular mass was determined by gel permeation chromatography. Sucrose phosphorylases are members of the glycosyl hydrolase family 13 [vandenBroek04]. The crystal structure of this enzyme has been refined at a resolution of 1.77 Å [Sprogoe04].
The molecular mass of the subunit was determined by SDS-PAGE [vandenBroek04].
Molecular Weight of Polypeptide: 56.19 kD (from nucleotide sequence), 58 kD (experimental) [vandenBroek04 ]
Molecular Weight of Multimer: 129 kD (experimental) [vandenBroek04]
Relationship Links: CAZy:IN-FAMILY:GH13 , Entrez-Nucleotide:PART-OF:AF543301 , InterPro:IN-FAMILY:IPR006047 , InterPro:IN-FAMILY:IPR013781 , InterPro:IN-FAMILY:IPR015325 , InterPro:IN-FAMILY:IPR015902 , InterPro:IN-FAMILY:IPR016377 , InterPro:IN-FAMILY:IPR017853 , InterPro:IN-FAMILY:IPR022527 , Panther:IN-FAMILY:PTHR10357 , PDB:Structure:1R7A , PDB:Structure:2GDU , PDB:Structure:2GDV , Pfam:IN-FAMILY:PF00128 , Pfam:IN-FAMILY:PF09244
Enzymatic reaction of: sucrose phosphorylase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: sucrose degradation IV (sucrose phosphorylase)
Sucrose phosphorylase reversibly catalyzes the phosphorolysis of sucrose. It also has transglucosylation activity [vandenBroek04].
T(opt): 48 °C [vandenBroek04]
pH(opt): 6.0-6.5 [vandenBroek04]
Sprogoe04: Sprogoe D, van den Broek LA, Mirza O, Kastrup JS, Voragen AG, Gajhede M, Skov LK (2004). "Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis." Biochemistry 43(5);1156-62. PMID: 14756551
vandenBroek04: van den Broek LA, van Boxtel EL, Kievit RP, Verhoef R, Beldman G, Voragen AG (2004). "Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083." Appl Microbiol Biotechnol 65(2);219-27. PMID: 14740189
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