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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: sucrose phosphorylase

Gene: sucP Accession Number: G-9706 (MetaCyc)

Species: Bifidobacterium adolescentis

Subunit composition of sucrose phosphorylase = [SucP]2
         sucrose phosphorylase subunit = SucP

Summary:
The recombinant enzyme was expressed in Escherichia coli and purified. The native molecular mass was determined by gel permeation chromatography. Sucrose phosphorylases are members of the glycosyl hydrolase family 13 [vandenBroek04]. The crystal structure of this enzyme has been refined at a resolution of 1.77 Å [Sprogoe04].
The molecular mass of the subunit was determined by SDS-PAGE [vandenBroek04].

Molecular Weight of Polypeptide: 56.19 kD (from nucleotide sequence), 58 kD (experimental) [vandenBroek04 ]

Molecular Weight of Multimer: 129 kD (experimental) [vandenBroek04]

Unification Links: Protein Model Portal:Q84HQ2 , SMR:Q84HQ2 , UniProt:Q84HQ2

Relationship Links: CAZy:IN-FAMILY:GH13 , Entrez-Nucleotide:PART-OF:AF543301 , InterPro:IN-FAMILY:IPR006047 , InterPro:IN-FAMILY:IPR013781 , InterPro:IN-FAMILY:IPR015325 , InterPro:IN-FAMILY:IPR015902 , InterPro:IN-FAMILY:IPR016377 , InterPro:IN-FAMILY:IPR017853 , InterPro:IN-FAMILY:IPR022527 , Panther:IN-FAMILY:PTHR10357 , PDB:Structure:1R7A , PDB:Structure:2GDU , PDB:Structure:2GDV , Pfam:IN-FAMILY:PF00128 , Pfam:IN-FAMILY:PF09244

Gene-Reaction Schematic: ?

Credits:
Created 18-Oct-2006 by Fulcher CA , SRI International


Enzymatic reaction of: sucrose phosphorylase

Synonyms: BiSP

EC Number: 2.4.1.7

sucrose + phosphate <=> β-D-fructofuranose + α-D-glucose 1-phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for sucrose: α-D-glucose 1-phosphate [vandenBroek04 ]

In Pathways: sucrose degradation IV (sucrose phosphorylase)

Summary:
Sucrose phosphorylase reversibly catalyzes the phosphorolysis of sucrose. It also has transglucosylation activity [vandenBroek04].

T(opt): 48 °C [vandenBroek04]

pH(opt): 6.0-6.5 [vandenBroek04]


References

Sprogoe04: Sprogoe D, van den Broek LA, Mirza O, Kastrup JS, Voragen AG, Gajhede M, Skov LK (2004). "Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis." Biochemistry 43(5);1156-62. PMID: 14756551

vandenBroek04: van den Broek LA, van Boxtel EL, Kievit RP, Verhoef R, Beldman G, Voragen AG (2004). "Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083." Appl Microbiol Biotechnol 65(2);219-27. PMID: 14740189


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc12.