MetaCyc Enzyme: sucrose phosphorylase

Gene: sucP Accession Number: G-9706 (MetaCyc)

Species: Bifidobacterium adolescentis

Subunit composition of sucrose phosphorylase = [SucP]2
         sucrose phosphorylase subunit = SucP

The recombinant enzyme was expressed in Escherichia coli and purified. The native molecular mass was determined by gel permeation chromatography. Sucrose phosphorylases are members of the glycosyl hydrolase family 13 [vandenBroek04]. The crystal structure of this enzyme has been refined at a resolution of 1.77 Å [Sprogoe04].
The molecular mass of the subunit was determined by SDS-PAGE [vandenBroek04].

Molecular Weight of Polypeptide: 56.19 kD (from nucleotide sequence), 58 kD (experimental) [vandenBroek04 ]

Molecular Weight of Multimer: 129 kD (experimental) [vandenBroek04]

Unification Links: Protein Model Portal:Q84HQ2 , SMR:Q84HQ2 , UniProt:Q84HQ2

Relationship Links: CAZy:IN-FAMILY:GH13 , Entrez-Nucleotide:PART-OF:AF543301 , InterPro:IN-FAMILY:IPR006047 , InterPro:IN-FAMILY:IPR013781 , InterPro:IN-FAMILY:IPR015325 , InterPro:IN-FAMILY:IPR015902 , InterPro:IN-FAMILY:IPR016377 , InterPro:IN-FAMILY:IPR017853 , InterPro:IN-FAMILY:IPR022527 , Panther:IN-FAMILY:PTHR10357 , PDB:Structure:1R7A , PDB:Structure:2GDU , PDB:Structure:2GDV , Pfam:IN-FAMILY:PF00128 , Pfam:IN-FAMILY:PF09244

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 18-Oct-2006 by Fulcher CA , SRI International

Enzymatic reaction of: sucrose phosphorylase

Synonyms: BiSP

EC Number:

sucrose + phosphate <=> β-D-fructofuranose + α-D-glucopyranose 1-phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for sucrose: α-D-glucopyranose 1-phosphate [vandenBroek04 ]

In Pathways: sucrose degradation IV (sucrose phosphorylase)

Sucrose phosphorylase reversibly catalyzes the phosphorolysis of sucrose. It also has transglucosylation activity [vandenBroek04].

T(opt): 48 °C [vandenBroek04]

pH(opt): 6.0-6.5 [vandenBroek04]


Sprogoe04: Sprogoe D, van den Broek LA, Mirza O, Kastrup JS, Voragen AG, Gajhede M, Skov LK (2004). "Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis." Biochemistry 43(5);1156-62. PMID: 14756551

vandenBroek04: van den Broek LA, van Boxtel EL, Kievit RP, Verhoef R, Beldman G, Voragen AG (2004). "Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083." Appl Microbiol Biotechnol 65(2);219-27. PMID: 14740189

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon Aug 3, 2015, biocyc14.