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MetaCyc Enzyme: thiamin phosphate synthase

Gene: thiE Accession Number: BSU38290 (MetaCyc)

Synonyms: thiC, ipa-26d, ywbK, thiamine-phosphate pyrophosphorylase, thiamin phosphate diphosphorylase

Species: Bacillus subtilis subtilis 168

Summary:
Thiamine-phosphate diphosphorylase (ThiE) catalyzes an important step in the de novo pathway of thiamin diphosphate biosynthesis, the coupling of the two moieties of thiamin, a thiazole (2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate) and a pyrimidine (4-amino-2-methyl-5-diphosphomethylpyrimidine), forming thiamin phosphate [Backstrom95].

In addition, the enzyme may catalyze a similar reaction in a slavage pathway, where it couples 4-methyl-5-(2-phosphonooxyethyl)thiazole to the pyrimidine moiety.

While most of the research on ThiE was performed with the Escherichia coli enzyme, a genetic screen designed to identify Bacillus subtilis genes that participate in thiamin metabolism identifed the thiE gene [Lawhorn04]. The crystal structure of the Bacillus subtilis enzyme complexed with the reaction products thiamin phosphate and diphosphate has been determined [Chiu99]. The structure and other mechanistic studies are consistent with a reaction mechanism involving the ionization of 4-amino-2-methyl-5-diphosphomethylpyrimidine at the active site to give the pyrimidine carbocation. The reaction is completed by trapping of the carbocation by the thiazole followed by product dissociation [Chiu99].

Map Position: [3,930,707 <- 3,931,375]

Molecular Weight of Polypeptide: 23.681 kD (from nucleotide sequence)

Unification Links: DBTBS Operons:thiE , Entrez:2636364 , GenoList (SubtiList):BSU38290 , GOA:P39594 , Protein Model Portal:P39594 , SMR:P39594 , String:224308.BSU38290 , String:BSU38290 , SubtilisWiki:thiE , SubtiWiki:thiE , UniProt:P39594

Relationship Links: InterPro:IN-FAMILY:IPR003733 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR022998 , PDB:Structure:1G4E , PDB:Structure:1G4P , PDB:Structure:1G4S , PDB:Structure:1G4T , PDB:Structure:1G67 , PDB:Structure:1G69 , PDB:Structure:1G6C , PDB:Structure:2TPS , PDB:Structure:3O15 , PDB:Structure:3O16 , Pfam:IN-FAMILY:PF02581

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
GO:0009228 - thiamine biosynthetic process Inferred by computational analysis [GOA00, GOA01a]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0004789 - thiamine-phosphate diphosphorylase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [GOA00]
GO:0046872 - metal ion binding Inferred by computational analysis [GOA00]

Gene Class: UNCLASSIFIED

Credits:
Imported from BsubCyc 20-Sep-2011 by Caspi R , SRI International
Revised 20-Sep-2011 by Caspi R , SRI International


Enzymatic reaction of: thiamin-phosphate diphosphorylase (thiamin phosphate synthase)

EC Number: 2.5.1.3

4-methyl-5-(2-phosphonooxyethyl)thiazole + 4-amino-2-methyl-5-diphosphomethylpyrimidine + H+ <=> thiamin phosphate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: thiamin salvage II


Enzymatic reaction of: thiamin-phosphate diphosphorylase (thiamin phosphate synthase)

EC Number: 2.5.1.3

2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2 H+ <=> thiamin phosphate + CO2 + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of thiamin diphosphate biosynthesis II , thiamin diphosphate biosynthesis II (Bacillus)

Credits:
Imported from BsubCyc 20-Sep-2011 by Caspi R , SRI International


References

Backstrom95: Backstrom AD, McMordie RAS, Begley TP (1995). "Biosynthesis of Thiamin I: The Function of the thiE Gene Product." J Am Chem Soc 117: 2351-2352.

Chiu99: Chiu HJ, Reddick JJ, Begley TP, Ealick SE (1999). "Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution." Biochemistry 38(20);6460-70. PMID: 10350464

GOA00: GOA (2000). "Gene Ontology annotation based on Swiss-Prot keyword mapping."

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Lawhorn04: Lawhorn BG, Gerdes SY, Begley TP (2004). "A genetic screen for the identification of thiamin metabolic genes." J Biol Chem 279(42);43555-9. PMID: 15292217


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC13B.