MetaCyc Enzyme: [sulfur-carrier protein ThiS] adenylyltransferase

Gene: thiF Accession Number: BSU11700 (MetaCyc)

Synonyms: yjbU, adenylate transferase and sulfur transferase

Species: Bacillus subtilis subtilis 168

The ThiF protein is involved in the posttranslational modification of ThiS by the conversion of the carboxylic acid group of the carboxyl-terminal glycine into a thiocarboxylate [Taylor98]. More specifically, ThiF catalyzes the adenylation of the carboxyl-terminal glycine of ThiS by ATP [Begley99, Taylor98]. ThiS and ThiF form a covalently linked protein-protein conjugate as an essential intermediate of this reaction [Xi01].

The thiF gene of Bacillus subtilis has been cloned, expressed in Escherichia coli and purified, and used in an in vitro reconstitution reaction for production of the thiazole moiety of thiamin phosphate [Park03]. When ThiS was incubated with ThiF and ATP, the formation of a carboxy-adenylated-[ThiS sulfur-carrier protein] was observed by ESI-FTMS. Unlike in Escherichia coli, no cross-linking between ThiF and ThiS has been detected [Park03].

Map Position: [1,245,808 -> 1,246,818]

Molecular Weight of Polypeptide: 36.4 kD (from nucleotide sequence)

Unification Links: DBTBS Operons:thiF , Entrez:2633524 , GenoList (SubtiList):BSU11700 , GOA:O31619 , HSSP:1JW9 , Mint:MINT-8367236 , Protein Model Portal:O31619 , SMR:O31619 , String:224308.BSU11700 , String:BSU11700 , SubtilisWiki:thiF , SubtiWiki:thiF , UniProt:O31619

Relationship Links: InterPro:IN-FAMILY:IPR000594 , InterPro:IN-FAMILY:IPR007901 , InterPro:IN-FAMILY:IPR009036 , InterPro:IN-FAMILY:IPR016040 , Pfam:IN-FAMILY:PF00899 , Pfam:IN-FAMILY:PF05237

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
GO:0009228 - thiamine biosynthetic process Inferred by computational analysis [GOA00]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [GOA00]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0005488 - binding Inferred by computational analysis [GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [GOA00]
GO:0016740 - transferase activity Inferred by computational analysis [GOA00]
GO:0016779 - nucleotidyltransferase activity Inferred by computational analysis [GOA00, GOA01]
GO:0046872 - metal ion binding Inferred by computational analysis [GOA00]


Imported from BsubCyc 15-Sep-2011 by Caspi R , SRI International
Revised 20-Sep-2011 by Caspi R , SRI International

Enzymatic reaction of: [sulfur-carrier protein ThiS] adenylyltransferase

EC Number:

a ThiS sulfur-carrier protein + ATP + H+ <=> a carboxy-adenylated-[ThiS sulfur-carrier protein] + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of thiamin diphosphate biosynthesis II , thiazole biosynthesis II (Bacillus)


Begley99: Begley TP, Downs DM, Ealick SE, McLafferty FW, Van Loon AP, Taylor S, Campobasso N, Chiu HJ, Kinsland C, Reddick JJ, Xi J (1999). "Thiamin biosynthesis in prokaryotes." Arch Microbiol 1999;171(5);293-300. PMID: 10382260

GOA00: GOA (2000). "Gene Ontology annotation based on Swiss-Prot keyword mapping."

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Park03: Park JH, Dorrestein PC, Zhai H, Kinsland C, McLafferty FW, Begley TP (2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)." Biochemistry 42(42);12430-8. PMID: 14567704

Taylor98: Taylor SV, Kelleher NL, Kinsland C, Chiu HJ, Costello CA, Backstrom AD, McLafferty FW, Begley TP (1998). "Thiamin biosynthesis in Escherichia coli. Identification of this thiocarboxylate as the immediate sulfur donor in the thiazole formation." J Biol Chem 273(26);16555-60. PMID: 9632726

Xi01: Xi J, Ge Y, Kinsland C, McLafferty FW, Begley TP (2001). "Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex." Proc Natl Acad Sci U S A 98(15);8513-8. PMID: 11438688

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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