|Gene:||DHFS||Accession Number: AT5G41480 (MetaCyc)|
Synonyms: At5g41480, AtDFA
Species: Arabidopsis thaliana col
The Arabidopsis genome was searched for sequences similar to dihydrofolate synthetase/folylpolyglutamate synthetase (DHFS/FPGS) signatures and four cDNA's were retrieved. The molecular functions of the cDNA's were tested by functional complementing mutant yeasts defect in either DHFS or FPGS activity. Protein extracts from the complemented yeast were used to confirm the specific function of the enzyme in vitro. One of the cDNA's, named AtDFA (At5g41480), encoded for the dihydrofolate synthetase (DHFS) in Arabidopsis thaliana catalyzing the conversion from 7,8-dihydropteroate to 7,8-dihydrofolate but did not exhibit any folypolylglutamate synthase activity [Ravanel01]. The mitochondrial location of the enzymes marks this compartment as the only one with a complete set of all enzymes involved in folate biosynthesis.
|Map Position: [16,613,155 -> 16,615,864]|
Molecular Weight of Polypeptide: 56.8 kD (from nucleotide sequence), 56.8 kD (experimental) [Ravanel01 ]
Relationship Links: Entrez-Nucleotide:PART-OF:AJ250872 , InterPro:IN-FAMILY:IPR001645 , InterPro:IN-FAMILY:IPR004101 , InterPro:IN-FAMILY:IPR013221 , InterPro:IN-FAMILY:IPR018109 , Panther:IN-FAMILY:PTHR11136 , Pfam:IN-FAMILY:PF08245 , Prosite:IN-FAMILY:PS01011 , Prosite:IN-FAMILY:PS01012
|Cellular Component:||GO:0005739 - mitochondrion [Ravanel01]|
Enzymatic reaction of: dihydrofolate synthetase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
Ravanel01: Ravanel S, Cherest H, Jabrin S, Grunwald D, Surdin-Kerjan Y, Douce R, Rebeille F (2001). "Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana." Proc Natl Acad Sci U S A 98(26);15360-5. PMID: 11752472
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