MetaCyc Enzyme: dihydrofolate synthetase

Gene: DHFS Accession Number: AT5G41480 (MetaCyc)

Synonyms: At5g41480, AtDFA

Species: Arabidopsis thaliana col

The Arabidopsis genome was searched for sequences similar to dihydrofolate synthetase/folylpolyglutamate synthetase (DHFS/FPGS) signatures and four cDNA's were retrieved. The molecular functions of the cDNA's were tested by functional complementing mutant yeasts defect in either DHFS or FPGS activity. Protein extracts from the complemented yeast were used to confirm the specific function of the enzyme in vitro. One of the cDNA's, named AtDFA (At5g41480), encoded for the dihydrofolate synthetase (DHFS) in Arabidopsis thaliana catalyzing the conversion from 7,8-dihydropteroate to 7,8-dihydrofolate but did not exhibit any folypolylglutamate synthase activity [Ravanel01]. The mitochondrial location of the enzymes marks this compartment as the only one with a complete set of all enzymes involved in folate biosynthesis.

Locations: mitochondrion

Map Position: [16,613,155 -> 16,615,864]

Molecular Weight of Polypeptide: 56.8 kD (from nucleotide sequence), 56.8 kD (experimental) [Ravanel01 ]

Unification Links: ArrayExpress:Q8W041 , Entrez:CAC80838 , PhylomeDB:Q8W041 , Pride:Q8W041 , Protein Model Portal:Q8W041 , SMR:Q8W041 , String:Q8W041 , TAIR:At5g41480 , UniProt:Q8W041

Relationship Links: Entrez-Nucleotide:PART-OF:AJ250872 , InterPro:IN-FAMILY:IPR001645 , InterPro:IN-FAMILY:IPR004101 , InterPro:IN-FAMILY:IPR013221 , InterPro:IN-FAMILY:IPR018109 , Panther:IN-FAMILY:PTHR11136 , Pfam:IN-FAMILY:PF08245 , Prosite:IN-FAMILY:PS01011 , Prosite:IN-FAMILY:PS01012

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005739 - mitochondrion [Ravanel01]

Revised 20-Jul-2010 by Pujar A , Boyce Thompson Institute

Enzymatic reaction of: dihydrofolate synthetase

EC Number:

L-glutamate + 7,8-dihydropteroate + ATP <=> ADP + 7,8-dihydrofolate monoglutamate + phosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of tetrahydrofolate biosynthesis , tetrahydrofolate biosynthesis

Citations: [Ravanel01]


Ravanel01: Ravanel S, Cherest H, Jabrin S, Grunwald D, Surdin-Kerjan Y, Douce R, Rebeille F (2001). "Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana." Proc Natl Acad Sci U S A 98(26);15360-5. PMID: 11752472

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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