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MetaCyc Enzyme: 10'-apo-β-carotenal 13,14-cleaving dioxygenase

Gene: CCD8 Accession Number: AT4G32810 (MetaCyc)

Synonyms: At4G32810

Species: Arabidopsis thaliana col

Summary:
The CCD8 gene is one of nine Arabidopsis thaliana genes that encode carotenoid dioxygenases. The gene encodes a 10'-apo-β-carotenal 13,14-cleaving dioxygenase, an enzyme that catalyzes a complex oxigenation/rearrangement of 9-cis-10'-apo-β-carotenal, generating carlactone, an intermediate leading to production of strigolactones [Alder12].

As typical to genes involved in this pathway, mutants in CCD8 display a dramatic increase in lateral branching [Sorefan03].

In an earlier work the protein was co-expressed with CCD7 in carotenoid-accumulating strains of Escherichia coli. It was found that the recombinant protein catalyzed the cleavage of all-trans-10'-apo-β-carotenal (produced by CCD7) to 13-apo-β-carotenone and a C9 dialdehyde [Schwartz04]. However, later work has shown that the in vivo substrate of the enzyme is 9-cis-10'-apo-β-carotenal, and that CCD8 alone is sufficient for conversion of this compound into a strigolactone-like compound [Alder12]. It was also shown that the reaction with 9-cis-10'-apo-β-carotenal is 10-fold faster than that with all-trans-10'-apo-β-carotenal [Alder12].

Unification Links: PhylomeDB:Q8VY26 , Pride:Q8VY26 , Protein Model Portal:Q8VY26 , SMR:Q8VY26 , String:Q8VY26 , TAIR:AT4G32810 , UniProt:Q8VY26

Relationship Links: InterPro:IN-FAMILY:IPR004294 , Panther:IN-FAMILY:PTHR10543 , Pfam:IN-FAMILY:PF03055

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 12-May-2011 by Zhang P , PMN
Revised 21-Aug-2012 by Caspi R , SRI International


Enzymatic reaction of: 9-cis-10'-apo-β-carotenal cleavage dioxygenase (10'-apo-β-carotenal 13,14-cleaving dioxygenase)

EC Number: 1.13.11.69

9-cis-10'-apo-β-carotenal + 2 oxygen <=> carlactone + (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: 5-deoxystrigol biosynthesis


Enzymatic reaction of: all-trans-β-apo-10'-carotenal cleavage dioxygenase (10'-apo-β-carotenal 13,14-cleaving dioxygenase)

EC Number: 1.13.11.70

all-trans-10'-apo-β-carotenal + oxygen <=> 13-apo-β-carotenone + (2E,4E,6E)-4-methylocta-2,4,6-trienedial

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: carotenoid cleavage

Summary:


References

Alder12: Alder A, Jamil M, Marzorati M, Bruno M, Vermathen M, Bigler P, Ghisla S, Bouwmeester H, Beyer P, Al-Babili S (2012). "The path from β-carotene to carlactone, a strigolactone-like plant hormone." Science 335(6074);1348-51. PMID: 22422982

Schwartz04: Schwartz SH, Qin X, Loewen MC (2004). "The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching." J Biol Chem 279(45);46940-5. PMID: 15342640

Sorefan03: Sorefan K, Booker J, Haurogne K, Goussot M, Bainbridge K, Foo E, Chatfield S, Ward S, Beveridge C, Rameau C, Leyser O (2003). "MAX4 and RMS1 are orthologous dioxygenase-like genes that regulate shoot branching in Arabidopsis and pea." Genes Dev 17(12);1469-74. PMID: 12815068


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue May 5, 2015, biocyc13.