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MetaCyc Polypeptide: MPT synthase large subunit

Gene: Cnx6 Accession Number: AT2G43760 (MetaCyc)

Synonyms: At2g43760

Species: Arabidopsis thaliana col

Component of: molybdopterin synthase (summary available)

Citations: [Mendel97, Mendel02, Gutzke01, Rudolph01, Rudolph03, Wuebbens03]

Molecular Weight of Polypeptide: 23.8 kD (from nucleotide sequence)

Unification Links: Entrez:CAB38428 , PhylomeDB:O22827 , Pride:O22827 , Protein Model Portal:O22827 , SMR:O22827 , String:3702.AT2G43760.3-P , TAIR:At2g43760 , UniProt:O22827

Relationship Links: Entrez-Nucleotide:PART-OF:AJ133519 , InterPro:IN-FAMILY:IPR003448 , Panther:IN-FAMILY:PTHR23404:SF2 , Pfam:IN-FAMILY:PF02391

Gene-Reaction Schematic: ?

Credits:
Created 04-Oct-2006 by Foerster H , TAIR


Subunit of: molybdopterin synthase

Species: Arabidopsis thaliana col

Subunit composition of molybdopterin synthase = [Cnx6]2[Cnx7]2
         MPT synthase large subunit = Cnx6
         MPT synthase small subunit = Cnx7 (summary available)

Summary:
The functional molybdopterin synthase (MTP synthase) is a heterotetramer that consists of 2 large subunits (Cnx6) and 2 small subunits (Cnx7) [Mendel97] [Mendel02]. The large subunit Cnx6 was identified based on sequence homology to its bacterial complement MoaE and the small subunit (Cnx7) using a yeast two-hybrid screen [Gutzke01]. The structure and function of the enzyme including the mechanism of the catalyzed reaction has been investigated in detail in bacteria [Rudolph01, Rudolph03, Wuebbens03].

Credits:
Created 03-Oct-2006 by Foerster H , TAIR


Enzymatic reaction of: molybdopterin synthase

EC Number: 2.8.1.12

cyclic pyranopterin phosphate + 2 a thiocarboxylated small subunit of molybdopterin synthase + H2O <=> 2 a small subunit of molybdopterin synthase + molybdopterin

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: molybdenum cofactor biosynthesis


References

Gutzke01: Gutzke G, Fischer B, Mendel RR, Schwarz G (2001). "Thiocarboxylation of molybdopterin synthase provides evidence for the mechanism of dithiolene formation in metal-binding pterins." J Biol Chem 276(39);36268-74. PMID: 11459846

Mendel02: Mendel RR, Hansch R (2002). "Molybdoenzymes and molybdenum cofactor in plants." J Exp Bot 53(375);1689-98. PMID: 12147719

Mendel97: Mendel RR (1997). "Molybdenum cofactor of higher plants: biosynthesis and molecular biology." Planta 203(4);399-405. PMID: 9421926

Rudolph01: Rudolph MJ, Wuebbens MM, Rajagopalan KV, Schindelin H (2001). "Crystal structure of molybdopterin synthase and its evolutionary relationship to ubiquitin activation." Nat Struct Biol 8(1);42-6. PMID: 11135669

Rudolph03: Rudolph MJ, Wuebbens MM, Turque O, Rajagopalan KV, Schindelin H (2003). "Structural studies of molybdopterin synthase provide insights into its catalytic mechanism." J Biol Chem 278(16);14514-22. PMID: 12571227

Suzuki06b: Suzuki M, Mark Settles A, Tseung CW, Li QB, Latshaw S, Wu S, Porch TG, Schmelz EA, James MG, McCarty DR (2006). "The maize viviparous15 locus encodes the molybdopterin synthase small subunit." Plant J 45(2);264-74. PMID: 16367969

Wuebbens03: Wuebbens MM, Rajagopalan KV (2003). "Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis." J Biol Chem 278(16);14523-32. PMID: 12571226


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, biocyc13.