Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
UDP-sugar hydrolaseInferred from experiment: ushA

Reaction Locations: periplasm (sensu Gram-negative Bacteria)

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Marked as unbalanced.

Enzyme Commission Primary Name: UDP-sugar diphosphatase

Enzyme Commission Synonyms: nucleosidediphosphate-sugar pyrophosphatase, nucleosidediphosphate-sugar diphosphatase, UDP-sugar hydrolase, UDP-sugar pyrophosphatase

Enzyme Commission Summary:
A divalent cation is required for activity. UDP-sugar is the best substrate, although other nucleoside-sugar diphosphates are used as substrates with similar Km values but much lower maximum velocities. Thus, this enzyme has a specificity distinct from that of ADP-sugar diphosphatase ( EC Some but not all enzymes of this class also appear to have 5′-nucleotidase (see EC activity.

Citations: [Glaser67 , Garrett89]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reactions of [a ribonucleoside 5'-monophosphate + H2O → a ribonucleoside + phosphate] (
i1: UMP + H2O → uridine + phosphate (

i2: CMP + H2O → cytidine + phosphate (

i3: AMP + H2O → adenosine + phosphate (

Instance reactions of [a 2'-deoxyribonucleoside 5'-monophosphate + H2O → a 2'-deoxynucleoside + phosphate] (
i4: dGMP + H2O → 2'-deoxyguanosine + phosphate (

i5: dAMP + H2O → 2'-deoxyadenosine + phosphate (

i6: dUMP + H2O → 2'-deoxyuridine + phosphate (

i7: dCMP + H2O → 2'-deoxycytidine + phosphate (

i8: dTMP + H2O → thymidine + phosphate (

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Garrett89: Garrett AR, Johnson LA, Beacham IR (1989). "Isolation, molecular characterization and expression of the ushB gene of Salmonella typhimurium which encodes a membrane-bound UDP-sugar hydrolase." Mol Microbiol 3(2);177-86. PMID: 2548058

Glaser67: Glaser L, Melo A, Paul R (1967). "Uridine diphosphate sugar hydrolase. Purification of enzyme and protein inhibitor." J Biol Chem 1967;242(8);1944-54. PMID: 5337593

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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