|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Transport Reactions → Transport Reactions of Unknown Mechanism|
|Reactions Classified By Substrate → Small-Molecule Reactions|
Reaction Locations: inner membrane (sensu Gram-negative Bacteria)
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
Mass balance status: Balanced.
The carbohydrate component of lipid III is synthesized on the cytoplasmic side of the membrane. Lipid III is then translocated to the periplasmic side of the membrane by the flippase, WzxE [Rick03] where assembly of the polysaccharide chains occurs by a block-polymerization mechanism [Rick03]. The polysaccharide chains are then transferred to an as yet unidentified glyceride acceptor to yield ECAPG. Completed ECAPG polymers are then incorporated into the exterior leaflet of the outer membrane [Rick03].
Rick03: Rick PD, Barr K, Sankaran K, Kajimura J, Rush JS, Waechter CJ (2003). "Evidence that the wzxE gene of Escherichia coli K-12 encodes a protein involved in the transbilayer movement of a trisaccharide-lipid intermediate in the assembly of enterobacterial common antigen." J Biol Chem 278(19);16534-42. PMID: 12621029
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