Escherichia coli K-12 substr. MG1655 Reaction: transport of N-acetyl-α-D-fucosyl-(1→4)-N-acetyl-β-D-mannosaminouronyl-(1→4)-N-acetyl-α-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsTransport ReactionsTransport Reactions of Unknown Mechanism
Reactions Classified By SubstrateSmall-Molecule Reactions

Transporters and Genes:
lipid III flippaseInferred from experiment: wzxE

Transport reaction diagram

Reaction Locations: inner membrane (sensu Gram-negative Bacteria)

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

The carbohydrate component of lipid III is synthesized on the cytoplasmic side of the membrane. Lipid III is then translocated to the periplasmic side of the membrane by the flippase, WzxE [Rick03] where assembly of the polysaccharide chains occurs by a block-polymerization mechanism [Rick03]. The polysaccharide chains are then transferred to an as yet unidentified glyceride acceptor to yield ECAPG. Completed ECAPG polymers are then incorporated into the exterior leaflet of the outer membrane [Rick03].

Gene-Reaction Schematic

Gene-Reaction Schematic


Rick03: Rick PD, Barr K, Sankaran K, Kajimura J, Rush JS, Waechter CJ (2003). "Evidence that the wzxE gene of Escherichia coli K-12 encodes a protein involved in the transbilayer movement of a trisaccharide-lipid intermediate in the assembly of enterobacterial common antigen." J Biol Chem 278(19);16534-42. PMID: 12621029

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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