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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
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Escherichia coli K-12 substr. MG1655 Reaction: Transport of N-acetyl-α-D-fucosyl-(1→4)-N-acetyl-β-D-mannosaminouronyl-(1→4)-N-acetyl-α-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol

Superclasses: Reactions Classified By Conversion Type Simple Reactions Transport Reactions Transport Reactions of Unknown Mechanism
Reactions Classified By Substrate Small-Molecule Reactions

Transporters and Genes:
lipid III flippase Inferred from experiment : wzxE

Reaction Locations: inner membrane (sensu Gram-negative Bacteria)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Summary:
The carbohydrate component of lipid III is synthesized on the cytoplasmic side of the membrane. Lipid III is then translocated to the periplasmic side of the membrane by the flippase, WzxE [Rick03] where assembly of the polysaccharide chains occurs by a block-polymerization mechanism [Rick03]. The polysaccharide chains are then transferred to an as yet unidentified glyceride acceptor to yield ECAPG. Completed ECAPG polymers are then incorporated into the exterior leaflet of the outer membrane [Rick03].

Gene-Reaction Schematic: ?


References

Rick03: Rick PD, Barr K, Sankaran K, Kajimura J, Rush JS, Waechter CJ (2003). "Evidence that the wzxE gene of Escherichia coli K-12 encodes a protein involved in the transbilayer movement of a trisaccharide-lipid intermediate in the assembly of enterobacterial common antigen." J Biol Chem 278(19);16534-42. PMID: 12621029


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc12.