Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
5-hydroxy-CTP diphosphataseInferred from experiment: nudG

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 2-hydroxy-dATP diphosphatase

Enzyme Commission Synonyms: NUDT1, MTH1, MTH2, oxidized purine nucleoside triphosphatase, (2'-deoxy) ribonucleoside 5'-triphosphate pyrophosphohydrolase

Enzyme Commission Summary:
The enzyme hydrolyses oxidized purine nucleoside triphosphates such as 2-hydroxy-dATP, thereby preventing their misincorporation into DNA. It can also recognize 8-oxo-dGTP and 8-oxo-dATP, but with lower efficiency (cf. EC, 8-oxo-dGTP diphosphatase) [Fujikawa99].

Citations: [Sakumi93a, Kakuma95, Sakai02a, Fujikawa01]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 19-Mar-2012 by Keseler I, SRI International


Fujikawa01: Fujikawa K, Kamiya H, Yakushiji H, Nakabeppu Y, Kasai H (2001). "Human MTH1 protein hydrolyzes the oxidized ribonucleotide, 2-hydroxy-ATP." Nucleic Acids Res 29(2);449-54. PMID: 11139615

Fujikawa99: Fujikawa K, Kamiya H, Yakushiji H, Fujii Y, Nakabeppu Y, Kasai H (1999). "The oxidized forms of dATP are substrates for the human MutT homologue, the hMTH1 protein." J Biol Chem 274(26);18201-5. PMID: 10373420

Kakuma95: Kakuma T, Nishida J, Tsuzuki T, Sekiguchi M (1995). "Mouse MTH1 protein with 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphatase activity that prevents transversion mutation. cDNA cloning and tissue distribution." J Biol Chem 270(43);25942-8. PMID: 7592783

Sakai02a: Sakai Y, Furuichi M, Takahashi M, Mishima M, Iwai S, Shirakawa M, Nakabeppu Y (2002). "A molecular basis for the selective recognition of 2-hydroxy-dATP and 8-oxo-dGTP by human MTH1." J Biol Chem 277(10);8579-87. PMID: 11756418

Sakumi93a: Sakumi K, Furuichi M, Tsuzuki T, Kakuma T, Kawabata S, Maki H, Sekiguchi M (1993). "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis." J Biol Chem 268(31);23524-30. PMID: 8226881

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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