|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → RNA-Reactions → tRNA-Reactions|
EC Number: 126.96.36.199
In Pathway: tRNA processing
Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Mass balance status: Marked as unbalanced.
Enzyme Commission Primary Name: tRNA nucleotidyltransferase
Enzyme Commission Synonyms: phosphate-dependent exonuclease, RNase PH, ribonuclease PH
Enzyme Commission Summary:
Brings about the final exonucleolytic trimming of the 3-terminus of tRNA precursors in Escherichia coli by a phosphorolysis, producing a mature 3-terminus on tRNA and nucleoside diphosphate. Not identical with EC 188.8.131.52 polyribonucleotide nucleotidyltransferase.
Cudny88: Cudny H, Deutscher MP (1988). "3' processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement." J Biol Chem 263(3);1518-23. PMID: 3275667
Deutscher88: Deutscher MP, Marshall GT, Cudny H (1988). "RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase." Proc Natl Acad Sci U S A 85(13);4710-4. PMID: 2455297
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