Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
anhydro-N-acetylmuramoyl-L-alanine amidaseInferred from experimentInferred by computational analysis: amiD
N-acetyl-anhydromuramyl-L-alanine amidaseInferred from experiment: ampD

In Pathway: anhydromuropeptides recycling

Supersedes EC numbers:,,

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: N-acetylmuramoyl-L-alanine amidase

Enzyme Commission Synonyms: acetylmuramyl-L-alanine amidase, N-acetylmuramyl-L-alanine amidase, N-acylmuramyl-L-alanine amidase, acetylmuramoyl-alanine amidase, N-acetylmuramic acid L-alanine amidase, acetylmuramyl-alanine amidase, N-acetylmuramylalanine amidase, murein hydrolase, N-acetylmuramoyl-L-alanine amidase type I, N-acetylmuramoyl-L-alanine amidase type II

Enzyme Commission Summary:
The enzyme hydrolyses the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Citations: [Herbold75, Herbold75a, Ghuysen69, Ward82]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Ghuysen69: Ghuysen JM, Dierickx L, Coyette J, Leyh-Bouille M, Guinand M, Campbell JN (1969). "An improved technique for the preparation of Streptomyces peptidases and N-acetylmuramyl-l-alanine amidase active on bacterial wall peptidoglycans." Biochemistry 8(1);213-22. PMID: 5777325

Herbold75: Herbold DR, Glaser L (1975). "Bacillus subtilis N-acetylmuramic acid L-alanine amidase." J Biol Chem 250(5);1676-82. PMID: 803507

Herbold75a: Herbold DR, Glaser L (1975). "Interaction of N-acetylmuramic acid L-alanine amidase with cell wall polymers." J Biol Chem 250(18);7231-8. PMID: 809432

Ward82: Ward JB, Curtis CA, Taylor C, Buxton RS (1982). "Purification and characterization of two phage PBSX-induced lytic enzymes of Bacillus subtilis 168: an N-acetylmuramoyl-L-alanine amidase and an N-acetylmuramidase." J Gen Microbiol 128(6);1171-8. PMID: 6126517

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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